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- 库存:
大量
- 英文名:
TUNEL BrightRed Apoptosis Detection Kit
- 供应商:
上海信裕生物科技有限公司
- 规格:
20 rxn/50 rxn/100 rxn

• 经过优化的TMR red-dUTP与未标记dNTP的比例,提高荧光检测的信噪比和灵敏度
• 专利的Bright因子,使得荧光更亮,抗淬灭能力更强
• 高活性的重组TdT酶保证荧光掺入效率
• 适用于细胞爬片、石蜡切片和冰冻切片


图1. 小鼠骨骼肌注射诱导剂后产生凋亡。
A. TUNEL BrightRed染色,箭头所指为凋亡细胞 B. Merge,蓝色为DAPI标记所有细胞。

本试剂盒采用TUNEL (TdT mediated dUTP Nick End Labeling)法,应用末端脱氧核糖核苷酸转移酶(TdT)在凋亡细胞断裂的DNA的3′-羟基(3'-OH)末端催化掺入四甲基罗丹明-脱氧三磷酸尿苷(TMR red-dUTP)。试剂盒对标记反应进行了优化,采用最佳比例的TMR red-dUTP和未标记dNTP进行3'-OH末端的核苷酸掺入,使得同一个断裂的DNA片段末端可以形成更长的“标记尾巴”。该“标记尾巴”减少了相邻掺入dNTP上标记基团的空间位阻,增加每个断裂片段上的荧光基团数目,降低荧光基团相邻后可能造成的聚集和淬灭,从而提高检测灵敏度,减少非特异性反应。试剂盒中的BrightRed Labeling Mix包含TMR red-dUTP以及专利的Bright因子。这种独特的小分子化合物可非共价的与TMR red结合,增强其稳定性,并使其信号放大,从而使得标记物更亮,抗淬灭能力更强。
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文献和实验Detection of Apoptosis by the TUNEL Assay
Apoptosis is the name given to a normal cell process—that of programmed cell death. As the term implies, this is normally a well controlled and highly regulated series of events leading to the elimination of an “unwanted” cell
Detection of Apoptosis by TUNEL Assay
Terminal deoxynucleotidyl transferase (T dT) dU TP N ick-E nd L abeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis. The method is based on the ability
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
, however, can not provide information regarding the histological localization at single cell level. This can be done by immunocytochemical method (A) or by enzymatic in situ labeling of apoptosis-induced DNA strand breaks (TUNEL) (B). Apoptosis was observed
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