万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
TUNEL FITC Apoptosis Detection Kit
- 供应商:
上海信裕生物科技有限公司
- 规格:
20 rxn/50 rxn/100 rxn

● 经过优化的FITC-12-dUTP与未标记dNTP的比例,提高荧光检测的信噪比和灵敏度
● 高活性的重组TdT酶保证荧光掺入效率
● 适用于细胞爬片、石蜡切片和冰冻切片


图1. 果蝇三龄幼虫视盘(eye disc)。凋亡诱导基因Hid在特异性启动子驱动下在果蝇视盘的远端诱导出大量凋亡细胞。
A. TUNEL 染色,标记凋亡细胞。B. ELAV染色,标记所有的感光细胞。C. Merge。

图2. 人睾丸组织切片经DNase I 处理。
A. TUNEL染色,标记凋亡细胞。B. PI染色,标记所有细胞核。C. Merge。

细胞凋亡的一个显著特点是细胞染色体的DNA被内源核酸内切酶有规律的降解成长度约为180-200bp的整倍数的片段。本试剂盒采用TUNEL法,应用末端脱氧核糖核苷酸转移酶(TdT)在凋亡细胞断裂的DNA的3′-羟基(3′-OH)末端催化掺入荧光素-12-脱氧三磷酸尿苷(FITC-12-dUTP)。FITC-12-dUTP标记的DNA可以用荧光显微镜直接观察或者用流式细胞仪定量。
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Detection of Apoptosis by the TUNEL Assay
Apoptosis is the name given to a normal cell process—that of programmed cell death. As the term implies, this is normally a well controlled and highly regulated series of events leading to the elimination of an “unwanted” cell
Detection of Apoptosis by TUNEL Assay
Terminal deoxynucleotidyl transferase (T dT) dU TP N ick-E nd L abeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis. The method is based on the ability
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
, however, can not provide information regarding the histological localization at single cell level. This can be done by immunocytochemical method (A) or by enzymatic in situ labeling of apoptosis-induced DNA strand breaks (TUNEL) (B). Apoptosis was observed
技术资料暂无技术资料 索取技术资料





