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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
HiScript 1st Strand cDNA Synthesis Kit
- 供应商:
上海信裕生物科技有限公司
- 规格:
50 rxn/100 rxn

• HiScript® Reverse Transcriptase提供可靠的逆转录表现
• 宽泛的模板量范围可成功逆转录从10 pg-5 μg总RNA
• 高效的合成能力:可合成长达10 kb的cDNA
• 针对不同下游应用可选择不同引物,合成的一链cDNA可广泛应用于分子克隆、杂交、PCR扩增及qPCR反应等
• 操作简便快捷

HiScript® Reverse Transcriptase是在M-MLV (Moloney Murine Leukemia virus) 基础上经过多点突变得到的新型逆转录酶。该酶能够有效合成长达10 kb的cDNA,适用于常规RNA、富含GC区域或具有复杂二级结构RNA模板的逆转录。HiScript® 一链cDNA合成试剂盒基于HiScript® Reverse Transcriptase,包含合成高质量第一链cDNA所需的所有成分,适合于后续的PCR、qPCR实验。
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文献和实验Protocol for First-strand cDNA Synthesis
℃for 10 minutes and then at 37℃for 60 minutes.Stop the reaction by heating at 70℃for 10 minutes. Chill on ice.NoteThe synthesized cDNA can be amplified by the PCR (see Protocols for PCR using Taq and Pfu DNA Polymerases) without intermediate phenol
of mRNA) or random hexamers; the kit used here contains both kinds); nucleotides for DNA synthesis (dNTPs); MgC2 and buffers required by the enzyme, which are all present in the 5x Reaction Mix. Materials * iScript cDNA Synthesis Kit (BIORAD
methylation analysis, PCR, and multiplex PCR. The purified total RNA is ready to use in any application, such as RT-PCR, real-time RT-PCR, differential display, cDNA synthesis, Northern, dot, and slot blot analyses, and microarray analysis. The kits form
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