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Human CRH / CRF ELISA Kit (Com

petitive EIA) - LS-F5352
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  • $875
  • LSBio
  • 12.35 - 1000 pg/ml
  • LS-F5352
  • 美国
  • 2025年07月15日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 样本

      血清/血浆/组织匀浆液

    • 库存

      1000

    • 适应物种

      Human

    • 应用

      科研使用

    • 检测方法

      ELISA

    • 检测范围

      12.35 - 1000 pg/ml

    • 供应商

      康朗生物

    • 规格

      96T

    Human CRH / CRF ELISA Kit (Competitive EIA) - LS-F5352
    Competition ELISA Platform Overview
    Catalog
    Size
    Price
    LS-F5352-1
    1 plate
    $875 USD
    Available Discounts
    10% off 5+ units


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    Product Description

    LS-F5352 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CRH / CRF in samples of Plasma and Serum. It is based upon a Competitive EIA assay principle and can be used to detect levels of CRH / CRF as low as 12.35 picograms per milliliter.
    About CRH / CRF
    Corticotropin-releasing hormone is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in this protein has been observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis.P06850  NM_000756 NP_000747.1

     CRH ElisaKit, CRF ElisaKit, Corticoliberin ElisaKit, Corticotropin-releasing factor ElisaKit

    More CRH / CRF Products
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    Specifications

    Type
     
    Competitive EIA (enzyme immunoassay) kit
    Target
     
    CRH / CRF
    Reactivity
     
    Human
    Specificity
     
    Corticotropin Releasing Hormone (CRH) No significant cross-reactivity or interference between Corticotropin Releasing Hormone (CRH) and analogs was observed.
    Manual
     

    Intended Sample Types
     
    Plasma, Serum
    Format
     
    96-Well Strip Plate
    Detection
     
    Colorimetric - 450nm (TMB)
    Measurement
     
    Quantitative
    Detection Range
     
    12.35 - 1000 pg/ml
    Sensitivity
     
    12.35 pg/ml
    Assay Time
     
    2.5 h
    Precision
     
    Intra-Assay: CV<10% Inter-Assay: CV<12%
    Storage
     
    Short term: 4°C; Long term: see manual.
    Quality Assurance
     
    Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
    Kit Components
     
    • Coated 96-well Strip Plate
    • Adhesive Plate Sealers
    • Standard (Lyophilized)
    • Sample Diluent
    • Detection Reagent A (Green)(Lyophilized)
    • Detection Reagent B (Red)
    • Assay Diluent A
    • Assay Diluent B
    • TMB Substrate
    • Stop Solution
    • Wash Buffer (30x concentrate)
    • Reagent Diluent

    Restrictions
     
    For research use only.
    Guarantee
     
    This elisa kit carries the LSBio 100% Guarantee.
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    Images

     

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    Competition ELISA Platform Overview
    Competition ELISA Platform Overview

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    图标文献和实验
    相关实验
    • Characterization of Corticotropin‐Releasing Factor (CRF) Receptors

      , 600 nM, 190 nM, 60 nM, 19 nM, 6 nM, 1.9 nM, 0.6 nM, and 0.19 nM):   agonists: rat/human corticotropin‐releasing (r/hCRF), ovine CRF (oCRF), sauvagine, urotensin I, urocortin

    • 【分享】ELISA原理

      的酶活力直接反映游离的酶标记物。均相EIA在临床检验中较少应用。非均相EIA需先进行游离的和结合的标记物的分离。如前所述,固相载体可用作一种分离手段。这种固相酶免疫测定方法在1971年最初建立时称为酶联免疫吸附剂测定(enzyme linked immunosorbent assay),简称ELISA,在国内有译作酶联免疫吸附试验或酶标,已习用。2、ELISA的原理和类型2.1. ELISA的原理ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。结合在固相载体表面的抗原或抗体仍保持其免疫

    • Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum

        Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators

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