- $265
- Ybscience
- Yb--2522
- 美国/德国/中国
- 2025年07月13日
- WB IHC IF IP ChIP
- Human Mouse RatOtHumaneRat (Wide Ratange)
- 详见说明书
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Histone H3K9me2 Antibody
- 抗体英文名：
Histone H3K9me2 Antibody
- 靶点：
详见说明书
- 浓度：
1.0mg/ml
- 应用范围：
WB IHC IF IP ChIP
- 宿主：
Human Mouse RatOtHumaneRat (Wide Ratange)
- 供应商：
上海钰博生物科技有限公司
- 库存：
大量
- 目录编号：
2522
- 抗原来源：
Human Mouse RatOtHumaneRat (Wide Ratange)
- 保质期：
1年
- 适应物种：
详见说明书
- 标记物：
详见说明书
- 克隆性：
多克隆
- 保存条件：
Store at -20°C
- 形态：
液体或冻干粉
- 亚型：
详见说明书
- 免疫原：
详见说明书
- 规格：
50μg/50μl, 100μg/100μl
-
- atalog No. : Yb--2522
- Size : 50μg/50μl, 100μg/100μl
-
- Mol Weight : 15kDa
- UniProt : Q16695
-
- Isotype : Rabbit IgG
- Clonality : Polyclonal
-
- Price : $265/100μg
- Availability : Ship next day
Category
- Primary Antibodies
- Secondary antibody
- Loading control / tag antibody
Details
Alternative Names:
HIST1H3J; H3/j ;H3FJ ; Histone H3.1; Histone H3/a; Histone H3/b;Histone H3/c; Histone H3/d; Histone H3/f;Histone H3/h ; Histone H3/I;HistoneH3/j ;Histone H3/k; Histone H3/l;HIST3H3;
Application:
WB IHC IF IP ChIP
Reactivity:
Human Mouse RatOtHumaneRat (Wide Ratange)
Dilution:
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200 IP 1:50-1:200 CHIP 1:50-1:200
 |
Western blot analysis of extracts of HeLa cellline and H3 protein expressed in E.coli., usingH3K9me2 antibody|Dot-blot analysis of all sorts of methylation peptides using H3K9me2 antibody|Immunofluorescence analysis of 293T cell using H3K9me2 antibody. Bl |
Purification:
Affinity-chromatography
Specificity:
Histone H3K9me2 Antibody detects endogenous levels of total Histone H3K9me2
Immunogen:
A synthesized peptide derived from human Histone H3K9me2
Description:
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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文献和实验PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
to chromatin, by its histone tail interaction or by other interactions, then you would not expect to see loss of binding upon peptide competition. A good positive control for the assay is to use H3K9 dimethylated peptide to compete HP1 from the pericentric
Chromatin Immunoprecipitation Protocol for Histone Modification
. This has been very successful when using antibodies that detect site specific histone lysine methylation (Peters et al . 2003, Martens et al . 2005). However, for each different antibody the optimal concentration should be tested and calibrated. The stringency of washing
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