- ￥1700 - 4000
- GeneTex
- 美国
- GTX130274
- 2025年07月09日
- WB, IHC-P, IHC-Fr
- Rabbit
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse CD31. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Mouse
- 目录编号：
GTX130274
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P, IHC-Fr
- 浓度：
0.94 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
CD31
- 抗体英文名：
CD31 antibody
- 抗体名：
CD31 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
CD31 antibody detects CD31 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse artery.
CD31 stained by CD31 antibody (GTX130274) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
CD31 antibody detects CD31 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
Green: CD31 stained by CD31 antibody (GTX130274) diluted at 1:placenta.
Red: beta Actin, a Cytoskeleton marker, stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with CD31 antibody (GTX130274) diluted at 1:500 and competitor's antibody (ab124432) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD31 antibody (GTX130274) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CD31 antibody detects CD31 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
CD31 stained by CD31 antibody (GTX130274) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Wang HK et al., Diab Vasc Dis Res 2021 (PMID:33557613)
Eric Song et al., J Exp Med 2021 (PMID:33433624)
Yeh LC et al., Int J Mol Sci 2020 (PMID:32131445)
Deng Q et al., Stem Cell Res Ther 2020 (PMID:32245517)
Zhu L et al., Carbohydr Polym 2020 (PMID:32475560)
Zheng J et al., J Ethnopharmacol 2019 (PMID:31654796)
Ishii N et al., Biol Reprod 2019 (PMID:31373359)
PS Sung et al., EMBO Mol Med 2023 (PMID:37211986)
Nishikawa S et al., Diagnostics (Basel) 2022 (PMID:36292158)
Meng-Jin Lin et al., Int J Mol Sci 2021 (PMID:34200896)
Gu X et al., Acta Biomater 2022 (PMID:35041901)
Hu JL et al., Cell Transplant 2021 (PMID:34538121)
Lin MJ et al., Pharmaceutics 2021 (PMID:34575535)
CD31 分子 CD31 常用单克隆抗体或代号： SG134,TM3,HEC- 75;(PECAM、EndoCAM) 主要表达细胞： Pt,M,G,B,NK,Tsub [AS] 分子质量(kDa)和结构： gp140,血小板GPⅡa(IgSF) 功 能： 嗜同性或嗜异性(与CD38互为受体)粘附,炎症,En功能 CD31 Aka
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
有好几个同仁发消息问CD31染色，刚工作比较忙，今天分享一下我做的是免疫荧光，也试做过免疫组化，都可以做出来抗体：小鼠抗CD31单克隆抗体 英国Abcam公司（CD31抗体说明书里面有说多聚甲醛会掩盖抗体，所以推荐速冻切片，也尝试过速冻切片，不使用多聚甲醛，但是这个技术个人掌握不好，所以还是用了多聚甲醛固定）二抗： 山羊抗小鼠488单克隆抗体 美国CST公司 特别说明：我是从同学那里得到的经验，可能绿光稍微好做些，之前做红光
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