Human CDH3 / P-Cadherin ELISA

Human CDH3 / P-Cadherin ELISA Kit (Sandwich ELISA) - LS-F24643

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LS-F24643-1
1 plate
$690 USD
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Popular CDH3 / P-Cadherin Elisa Kits

Mouse CDH3 / P-Cadherin ELISA Kit (Sandwich ELISA) - LS-F24256
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Human CDH3 / P-Cadherin ELISA Kit (Sandwich ELISA) - LS-F2614
Type: Quantitative Sandwich ELISA
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Product Description

LS-F24643 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CDH3 / P-Cadherin in samples of Cell Culture Supernatants and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of CDH3 / P-Cadherin as low as 2 picograms per milliliter.
About CDH3 / P-Cadherin
CDH3 / P-Cadherin is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. Uniprot: P22223 NCBI: NM_001793 NP_001784.2

Synonyms: CDH3 ElisaKit, Cadherin-3 ElisaKit, HJMD ElisaKit, P-cadherin ElisaKit, CDHP ElisaKit, PCAD ElisaKit, Placental cadherin ElisaKit

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Specifications

Type
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
CDH3 / P-Cadherin
Reactivity
 
Human
Specificity
 
For the detection and quantification of endogenous levels of natural and/or recombinant Human P Cadherin proteins.
Manual
 

Intended Sample Types
 
Cell Culture Supernatants, Serum
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
62.5 - 4000 pg/ml
Sensitivity
 
2 pg/ml
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• Coated 96-well Strip Plate
• Standard (Lyophilized)
• Biotinylated Detection Antibody (100x)
• Avidin-Biotin-HRP Complex (100x)
• Sample Diluent
• Biotinylated Detection Antibody Diluent
• Avidin-Biotin-HRP Complex Diluent
• Wash Buffer (20x)
• TMB Substrate
• Stop Solution
• Adhesive Plate Sealers

Background
 
Cadherins are calcium-dependent cell-cell adhesion molecules that mediate cell-cell binding in a homophilic manner. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherin expression is regulated spatially as well as temporally. Cadherins are thought to play an important role in development and maintenance of tissues through selective cell-cell adhesion activity and may be involved also in the invasion and metastasis of malignant tumors. Cadherin regulates dendritic spine morphogenesis. A cadherin gene cluster is mapped to a region of chromosome 5 subject to frequent allelic loss in carcinoma.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio 100% Guarantee.
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