- $990
- LSBio
- 0.781 - 50 ng/ml
- LS-F33433
- 美国
- 2025年07月13日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Human
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
0.781 - 50 ng/ml
- 供应商：
康朗生物
- 规格：
96T
Human JUN / c-Jun ELISA Kit (Sandwich ELISA)
Print This Page
Technical Help
Review This Product
SpecificationsPublicationsReviewsImages
Product Description
LS-F33433 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human JUN / c-Jun in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of JUN / c-Jun as low as 0.469 nanograms per millilter.Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
JUN / c-Jun
Reactivity
Human
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.781 - 50 ng/ml
Sensitivity
0.469 ng/ml
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
Store at 4°C. Stable for 6 months.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Background
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- JUNantibody was pre-coated onto 96-well plates. And the biotin conjugated anti- JUN antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the JUN amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of JUN can be calculated.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About JUN / c-Jun
P05412 NM_002228 NP_002219.1
Popular JUN / c-Jun Elisa Kits
Mouse/Human/Rat JUN / c-Jun ELISA Kit (DNA-Binding ELISA) - LS-F732
Reactivity: Mouse, Human, Rat
Mouse/Human/Rat Phospho-JUN / c-Jun (Ser249) ELISA Kit (DNA-Binding Phosphorylation ELISA) - LS-F846
Reactivity: Mouse, Human, Rat
Mouse/Human/Rat Phospho-JUN / c-Jun (Ser63) ELISA Kit (DNA-Binding Phosphorylation ELISA) - LS-F847
Reactivity: Mouse, Human, Rat
Human Phospho-JUN / c-Jun (Ser73) ELISA Kit (DNA-Binding Phosphorylation ELISA) - LS-F848
Reactivity: Human
Mouse/Human/Rat Phospho-JUN / c-Jun (Ser243) ELISA Kit (DNA-Binding Phosphorylation ELISA) - LS-F849
Reactivity: Mouse, Human, Rat
More JUN / c-Jun Products
Publications (0)
Submit a Publication
Customer Reviews (0)
Submit a Review
Top of Page
Images
Sandwich ELISA Platform Overview
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验ELISA原理与实验方法The advantages of the ELISA are similar to other antibody-labeled reactions which include specificity, sensitivity, inexpensiveness, and safety. Since the enzyme label is the critical portion of ELISA, its selection is very important
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 and Total-p38 antibodies (C). Cell-Based Elisa将小鼠巨噬细胞4/4细胞种在96孔板内，每个孔种5 x 104 cells/cm2细胞。细胞用不同浓度的anisomycin刺激，我们来监测P38 MAPK和JNK两种蛋白的变化。按照试剂盒的实验步骤，分别固定，打孔，猝灭，封闭，然后一抗孵育，二抗孵育，最后显色，用常规的酶标仪读取数值。最后数据再和试剂盒提供的结晶紫核染料结果做纠正，最后得出以上数据。Quantitative
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
技术资料暂无技术资料 索取技术资料
