Recombinant Mouse Pro-EGF (aa

Mouse keratinocyte cultures

0.5 ml of 5 mg/ml insulin per 500 ml bottle of EMEM (final conc’n = 5μg/ml). Epidermal Growth Factor (EGF) Recombinant human EGF; GibcoBRL, cat # 13247-051 Prepare a 10μg/ml stock: Dissolve 100μg EGF in 1 ml 0.1% BSA (10 mg ultrapure BSA

Assessment of Insulin Secretion in the Mouse

I. Mature rat insulin I and II are identical except that Ser for Pro 9 and Met for Lys 29 substitutions are incorporated into the B-chain of rat insulin II. Relative to rat insulin I, mature human insulin contains substitutions of Asn

Mouse aortic ring assay: A new approach of the molecular genetics of angiogenesis

, we have adapted to the mouse, the aortic ring assay initially developed in the rat. The use of deficient mice allowed us to demonstrate that PAI-1 is essential for angiogenesis while the absence of an MMP, MMP-11, did not affect vessel sprouting. We report