- $265
- Ybscience
- Yb--3298
- 美国/德国/中国
- 2025年07月13日
- WB IP
- Mouse
- 详见说明书
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
EGF Receptor Mouse mAb
- 抗体英文名：
EGF Receptor Mouse mAb
- 靶点：
详见说明书
- 浓度：
1.0mg/ml
- 应用范围：
WB IP
- 宿主：
Mouse
- 供应商：
上海钰博生物科技有限公司
- 库存：
大量
- 目录编号：
3298
- 抗原来源：
Mouse
- 保质期：
1年
- 适应物种：
详见说明书
- 标记物：
详见说明书
- 克隆性：
多克隆
- 保存条件：
Store at -20°C
- 形态：
液体或冻干粉
- 亚型：
详见说明书
- 免疫原：
详见说明书
- 规格：
50μg/50μl, 100μg/100μl
-
- Catalog No. : Yb--3298
- Size : 50μg/50μl, 100μg/100μl
-
- Mol Weight : 175KDa
- UniProt : P00533
-
- Isotype : Mouse IgG1
- Clonality : Monoclonal
-
- Price : $265/100μg
- Availability : 3-4 days
Category
- Primary Antibodies
- Secondary antibody
- Loading control / tag antibody
Details
Alternative Names:
EC 2.7.10.1; Receptor protein-tyrosine kinase ErbB-1; kinase EGFR; ERBB1; Epidermal growth factor receptor precursor
Application:
WB IP
Reactivity:
Human
Dilution:
WB 1:2000 IP
 |
Western blot detection of EGFR in A549 cell lysates using EGFR mouse mAb(dilution 1:2000).Predicted band size:134 Kda.Observed band size:175KDa. |
 |
Immunoprecipitation analysis of Hela cell lysates using EGFR mouse mAb. |
Purification:
Affinity-chromatography
Specificity:
EGF Receptor Mouse mAb detects endogenous levels of total EGF Receptor
Immunogen:
Purified recombinant human EGFR protein fragments expressed in E.coli.
Description:
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues.
Storage Condition and Buffer:
Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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文献和实验EGF受体是一种糖蛋白, 广泛分布于哺乳动物的上皮细胞、人的成纤维细胞、胶质细胞、角质细胞等。EGF 受体是一条含有1186个氨基酸残基的多肽链, 相对分子质量为170kDa,由三个部分组成:①很大的细胞外结构域：约621个氨基酸残基，富含半胱氨酸(51个), 并形成多对二硫键，其上结合有糖基,是EGF结合的位点。②跨膜区∶由23个氨基酸残基组成;③细胞质结构域,由542个氨基酸残基组成,含有无活性的酪氨酸激酶和几个酪氨酸磷酸化的位点。
A Historical Perspective of the EGF Receptor and Related Systems
Since the isolation of epidermal growth factor (EGF) from mouse submaxillary glands in the early 1950s by Cohen and coworkers, this growth factor has been shown to have various effects on numerous cellular systems. The biological
Assays to Monitor Degradation of the EGF Receptor
to conduct receptor downregulation assays are described. Monitoring of ubiquitination and degradation of the EGFR subsequent to stimulation with the receptor ligand EGF is described. Finally, protocols to quantitatively measure degradation of the EGFR
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