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- 英文名:
Tryptose Phosphate Broth, Bacto™ Buffered Medium
- 规格:
2 kg
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文献和实验Vybrant® DyeCycle Green and Orange Stains
-cell leukemia line, in complete RPMI medium containing 10% fetal bovine serum with staining at 37?C, but can be adapted to most cell types. These stains can also be used for cells suspended in Hanks’ Balanced Salt Solution (HBSS) or phosphate-buffered
instead. The medium of some lymphoid cells is supplemented with 5×10-5 M 2-mercaptoethanol. It does not matter whether this has undergone oxidation. Some cells benefit from the inclusion of 2 mM glutamine, 2, 5 or 10% tryptose phosphate broth, 2 mM pyruvate, or non
, 2, 5 or 10% tryptose phosphate broth, 2 mM pyruvate, or non-essential amino acids in their media. For example, Mary-Ann Campbell always puts pyruvate and non-essential amino acids, in addition to mercaptoethanol, in the medium of her B cells.
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