ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BAF190A; Mitotic growth and transcription activator; Protein BRG-1; Protein brahma homolog 1; SNF2-beta;
Application:
WB ICC/IF IP
Reactivity:
Human,Mouse
Dilution:
WB 1:1000 ICC/IF 1:50 IP
Western blot detection of BRG1 in Hela NE,F9 and K562 cell lysates using BRG1 mouse mAb (1:1000 diluted).Predicted band size:220KDa.Observed band size:220KDa.
Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-BRG1 mouse mAb (dilution 1:50). DAPI was used to stain nucleus(blue).
Immunoprecipitation analysis of K562 cell lysates using BRG1 mouse mAb (201025).
Purification:
Affinity-chromatography
Specificity:
BRG1 Mouse mAb detects endogenous levels of total BRG1
Immunogen:
Purified recombinant human BRG1 protein fragments expressed in E.coli.
Description:
Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation.Component of the CREST-BRG1 complex,a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex.
Storage Condition and Buffer:
Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.