小鼠促血小板生成素elisa试剂盒，TPO elisa ki

Product name:

Mouse Thrombopoietin ELISA Kit

Tests:

1 X 96 well plate

Sample type:

Serum, Plasma

Assay type:

Sandwich

Sensitivity:

8.0 pg/mL

Range:

62.5-4000 pg/mL

Reacted Species:

Mouse

Tested applications:

Sandwich ELISA

Recovery:

Sample Type	Average	Range
Mouse serum	85%	78%-97%

IntraAssay:

Sample	n	mean (pg/mL)	SD	CV%
1	20	1,961.9	55.0	2.8
2	20	467.5	13.5	2.9
3	20	92.0	5.0	5.4

InterAssay:

Sample	n	mean (pg/mL)	SD	CV%
1	24	1,891.6	42.2	2.2
2	24	457.5	11.5	2.5
3	24	110.5	4.4	3.9

Product overview:

KE10059 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse Thrombopoietin ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Thrombopoietin. The assay recognizes mouse Thrombopoietin. An antibody specific for mouse Thrombopoietin has been pre-coated onto the microwells. The mouse Thrombopoietin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse Thrombopoietin is added to detect the captured mouse Thrombopoietin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Storage Instructions:

All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

Synonyms:

C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, MGDF, Myeloproliferative leukemia virus oncogene ligand, Thrombopoietin, Tpo