重组人转化生长因子β3，TGFB3 (Mammalian)

重组人转化生长因子β3，TGFB3 (Mammalian)

Recombinant Human/Mouse/Rat TGFB3 (CJ44)

产品说明(Description)

Recombinant Human/Mouse/Rat Transforming Growth Factor beta 3 is produced by our Mammalian expressionsystem and the target gene encoding Ala301-Ser412(Tyr340Phe) is expressed.

Accession #: P10600

Known as: Transforming growth factor beta-3;TGFB3;TGF-beta-3;Latency-associated peptide;LAP

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 50mM Glycine-HCl, 150mM NaCl, pH2.5.

质量控制(Quality Control)

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Bioactivity:

Measured by its ability to inhibit the IL-4-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 10-80 pg/ml.

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.

Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

背景(Background)

Transforming growth factor beta 3(TGFB3) is a member of a TGF -β superfamily which is defined by theirstructural and functional similarities. TGFB3 is secreted as a complex with LAP. This latent form of TGFB3 becomes active upon cleavage by plasmin, matrix metalloproteases, thrombospondin -1, and a subset ofintegrins. It binds with high affinity to TGF- β RII, a type II serine/threonine kinase receptor. TGFB3 is involved incell differentiation, embryogenesis and development.It is believed to regulate molecules involved in cellularadhesion and extracellular matrix (ECM) formation during the process of palate development. Without TGF-β3, mammals develop a deformity known as a cleft palate.