- ¥2570
- peprotech
- 400-36
- ProSpec
- 2025年09月27日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海研卉生物科技有限公司
- 库存：
3周
- 英文名：
Recombinant Rat Oncostatin M
- 规格：
10μg
Product Details
Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. Produced by activated T cells, monocytes and Kaposi’s sarcoma cells, OSM can exert both stimulatory and inhibitory effects on cell proliferation. It stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposi’s sarcoma cells, but inhibits the growth of some normal and tumor cell lines. It also promotes cytokine release (e.g. IL-6, GM-CSF and G-CSF) from endothelial cells, and enhances the expression of low-density lipoprotein receptors in hepatoma cells. OSM shares several structural and functional characteristics with LIF, IL-6, and CNTF. Recombinant Rat Oncostatin M is a 24.4 kDa protein, containing 215 amino acid residues.
Source: E.coli
Synonyms: OSM
AA Sequence: MKRGCSSSSP KLLSQLKSQA NITGNTASLL EPYILHQNLN TLTLRAACTE HPVAFPSEDM LRQLSKPDFL STVHATLGRV WHQLGAFRQQ FPKIQDFPEL ERARQNIQGI RNNVYCMARL LHPPLEIPEP TQADSGTSRP TTTAPGIFQI KIDSCRFLWG YHRFMGSVGR VFEEWGDGSR RSRRHSPLWA WLKGDHRIRP SRSSQSAMLR SLVPR
Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity: Assay #1:Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 is 3.0-5.0 μg/ml.
Assay #2: Determined by its ability to inhibit Alkaline phosphatase activity of differentiated MC3T3 E1 cells.
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文献和实验developed for in vitro studies (4 ). Recently, we have refined this approach to apply to in vivo studies of acute CNS injury (5 ,6 ). This technique allows for differential and concurrent measurement of the two major isoforms of calpain, m-calpain and μ
M-Current Recording from Acute DRG Slices
from rat dorsal root ganglion (DRG) slices.
These protocols should yield enough cytosol and organelles for 1-200 MT/Organelle motility assays. Solutions and Reagents • Freshly removed or flash frozen rat liver • PBS •Homogenization Buffer •Homogenization Buffer containing 0.5 mM mM
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