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- Biorbyt
- orb446673
- 2025年12月19日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商：
上海研卉生物科技有限公司
- 库存：
现货
- 抗体英文名：
TM4SF3 antibody (Biotin)
- 抗体名：
TM4SF3 antibody (Biotin)
- 规格：
100ug
| Product Name | TM4SF3 antibody (Biotin) |
|---|---|
| Catalog Number | orb446673 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Biotin |
| Tested applications | ELISA, ICC, IF, IHC-P, WB |
| Immunogen | KLH conjugated synthetic peptide derived from human TM4SF3 |
| Target | TM4SF3 (Biotin) |
| Form/Appearance | Liquid: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
|---|---|
| Concentration | - 100 μg (in 100 μl): 1 mg/ml |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Affinity purified by Protein A |
| MW | 26 kDa |
| Uniprot ID | P19075 (Human) |
| Entrez | 7103 |
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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