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- 文献和实验
- 技术资料
- 保修期:
PTEP24H48
- 库存:
大量
- 规格:
48个/盒
密理博8um小室,PET膜,24孔板
PTEP24H48 MilliporeMillicell Hanging Cell Culture Insert, PET 8 µm, 24-well, 48/pk
| Replacement Information | |
|---|---|
| Replacement Information | Replacement for MCEP24H48 |
| Description | |
|---|---|
| Catalogue Number | PTEP24H48 |
| Replaces | MCEP24H48 |
| Trade Name |
|
| Description | Millicell Hanging Cell Culture Insert, PET 8 µm, 24-well, 48/pk |
| Background Information | With Millicell inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell inserts make it possible to study both sides of the cell monolayer. Millicell inserts are available for 24-, 12-, or 6-well plates. The inserts are easily prepared for SEM and TEM visualizing techniques, and they are compatible with cellular and/or fluorescent stains. Insert Formats: Millicell Hanging Inserts: Preloaded in receivers or available standalone - For co-culturing and permeability assays - Unique design allows easier basolateral access than other hanging inserts with less risk of contamination |
| Product Information | |
|---|---|
| Quality Level | MQ200 |
| Applications | |
|---|---|
| Application | Polyethylene Terephthalate hanging cell culture insert with pore size of 8.0 µm used in a 24-well plate for cell attachment, cell culture, cell differentiation & ICC |
| Key Applications |
|
| Packaging Information | |
|---|---|
| Material Size | 48/box |
| Material Package | Individually blister packaged |
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文献和实验相关实验
肿瘤细胞侵袭试验(Tumour Invasion Assay)
法是在Transwell小室吊篮式上腔的滤膜上铺上30ugMartrigel,加入细胞72h后观察结果。值得注意的是,细胞在TransWll腔中培养72小时后,有相当数量穿过滤膜的细胞不再粘附在滤膜下表面,而是脱落进人下腔溶液中.因此统计穿过基质膜的细胞数目时应把这部分细胞考虑在内。肿瘤细胞穿过重建基质膜的能力与它的体内侵袭转移能力表现出较好的相关性,可以用重建基质膜模型初筛抗侵袭药物。在上述分析中,如果不在膜上铺Martrigel而直接将8um孔径滤膜安放在侵袭腔室上下腔室之间,细胞通过变形运动穿过滤膜,用这种模型
transwell小室侵袭实验? 问:我是用7901胃癌,用那种塑料的,就一个大孔的那种transwell。请问怎么做呢? 答:贴壁的胃癌7901细胞作起来更简单啊,只要你的材料齐全质量好就可以。 材料:侵袭小室(TranswellChanber 孔径8um),人工基质胶(Matrigel) 具体方法:设置加药感染7901胃癌细胞实验组及空白细胞对照组,24h后收取细胞进行实验。同时将Matrigel 40μl均匀的铺在TranswellChanber膜上,37℃ 30min成胶。取对照组和实验组细胞
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密理博8um小室,PET膜,24孔板
¥1600





