Human Caspase 1 ELISA Kit

Product name:

Human Caspase 1 ELISA Kit

Tests:

1 X 96 well plate

Sample type:

Serum, Plasma, Cell cuture supernatants

Assay type:

Sandwich

Sensitivity:

1.0 pg/mL

Range:

7.8-500 pg/mL

Reacted Species:

Human

Tested applications:

Sandwich ELISA

Recovery:

Sample Type	Average	Range
Human serum	84%	77%-92%
Cell cuture supernatants	100%	92%-114%

IntraAssay:

Sample	n	mean (pg/mL)	SD	CV%
1	20	197.4	6.2	3.1
2	20	46.3	1.5	3.3
3	20	10.2	0.7	7.0

InterAssay:

Sample	n	mean (pg/mL)	SD	CV%
1	24	198.0	12.1	6.1
2	24	50.1	4.2	8.4
3	24	11.9	0.9	7.9

Product overview:

KE00231 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Caspase 1 ELISA kit is to be used to detect and quantify protein levels of endogenous Caspase 1. The assay recognizes human Caspase 1. An antibody specific for Caspase 1 has been pre-coated onto the microwells. The Caspase 1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for human Caspase 1 is added to detect the captured human Caspase 1 protein. For signal development, HRP-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Storage Instructions:

All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

Synonyms:

CASP 1, CASP1, Caspase 1, Caspase1, ICE, IL 1 beta converting enzyme, IL 1BC, IL1BC, IL1BCE, Interleukin 1 beta convertase, P45