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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
CA 125 Antibody Mouse Monoclonal
- 库存:
大量
- 供应商:
上海研卉生物科技有限公司
- 规格:
6ml
Application
|
IHC |
|---|---|
| Primary Accession | Q8WXI7 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone Names | 765H4D5 |
| Gene ID | 94025 |
|---|---|
| Gene Name | MUC16 (HGNC:15582) |
| Other Names | Mucin-16, MUC-16, Ovarian cancer-related tumor marker CA125, CA-125, Ovarian carcinoma antigen CA125, MUC16 (HGNC:15582) |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CA 125 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Function | Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. |
|---|---|
| Cellular Location | Cell membrane; Single-pass type I membrane protein. Secreted, extracellular space. Note=May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain |
| Tissue Location | Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas |
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文献和实验上皮及间皮类常用标记抗体EPITHELIAL AND MESOTHELIAL MARKERSCK (MNF 116)- MNF-116 is a pan-cytokeratin antibody.- Source: Dakopatts.- Dilution 1:50.- Antigen retrieval method: HIER (pressure cooking) using EDTA.- Staining pattern: Cytoplasmic, typically
多是多克隆;而小鼠来源的抗体多是单克隆,但也有另外。这条主要要与后面的二抗来源相匹配。(3)实验目的是检测什么种属的抗原,即species reactivity。这一点很重要,一般说明书上都有注明,如小鼠Ms、大鼠Rat、人Hum等等。(4)能否做免疫组化。一般一抗说明书都会注明做WB、IHC、ICC、IF等,建议最好选择注明的抗体,因为一般都是经过文章证明。(5)检测标本类型。用于检测石蜡切片还是冰冻切片,一般能做石蜡切片的抗体,可能都可以用来检测冰冻切片,但能做冰冻切片的抗体,不一定能检测石蜡
肺部肿瘤常用免疫组化抗体: CK7 细胞角蛋白7 阳性部位:胞浆 细胞角蛋白7(CK7)分子量为54KDa,主要标记腺上皮和移行上皮,卵巢,肺和乳腺上皮CK7阳性,而结肠、前列腺和胃肠道上皮为CK7阴性,因此可用于肺癌(CK7+)与结肠癌(CK7-)的鉴别。 CK20 细胞角蛋白20 阳性部位:胞浆 细胞角蛋白20(CK20)的分子量为46KDa,主要标记胃肠道上皮、尿道
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