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上海研卉生物科技有限公司
- 规格：
100g
Sephadex G-50 Medium
Sephadex G-50 resin is well established gel filtration resin for desalting and buffer exchange of biomolecules >30 000 molecular weight. The larger bead size of G-50 resin is suited for large scale separations.
Sephadex G-50 resin is well established gel filtration resin for desalting and buffer exchange of biomolecules >30 000 molecular weight. The larger bead size of G-50 resin is suited for large scale separations.
- Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
- Suitable for purification of DNA from small molecules by gel filtration
- Larger bead size for large scale group separations where high flow rates and low operating pressures are required.
- Classic gel filtration resin.
Sephadex is a gel filtration resin prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, resin, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.
| Parameter | Sephadex G-50 Medium, 100 g |
|---|---|
| Matrix | Cross-linked dextran |
| Particle size distribution, wet | 100–300 µm |
| Particle Size Dry | 50 µm-150 µm |
| Application | For the separation of molecules Mr >30 000 from molecules Mr <1500 such as labeled protein or DNA from unconjugated dyes For removeing small nucleotides from longer chain nucleic acids |
| pH stability, operational1 | 2–10 |
| pH stability, CIP2 | 2–13 |
| Flow Rate3 | < Darcy´s Law |
| Pressure/Flow Specification | min 150 cm/h, bed height 10 cm, column 5 cm i.d. |
| Fractionation range [Mr], Globular proteins | 1 × 10³–3 × 10⁴ |
| Fractionation [Mp] Dextrans | 5 x 10²-1 x 10⁴ |
| Exclusion Limit | > 3 x 10⁴ |
| Storage | 4 to 30°C |
| Autoclavable | In wet form (pH 7.0) at 120°C for 30 min |
上海研卉生物科技有限公司 ，是一家专注于生命科学和生物技术领域的高科技企业，专业提供分子生物学、免疫学、生命科学基础研究以及临床检测等诸多领域的试剂、耗材、仪器等各类产品及生物技术服务。公司主营业务： 试剂盒：酶联免疫试剂盒、免疫印迹试剂盒、免疫组化试剂盒、细胞凋亡检测试剂盒、分子生物学试剂盒。 细胞：原装进口细胞、国产肿瘤细胞、国产正常细胞。 耗材：细胞培养耗材、普通实验耗材。 生化试剂：原装进口生化试剂、进口分装生化试剂、国产生化试剂。 抗体：免疫组化、免疫荧光、流式细胞检测、免疫细胞化学。
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文献和实验Sephadex G-50 spun column purification
Sephadex G-50 spun column purification Spin column purification can be used to change buffers without a concomitant change in solution volume, to remove protein contaminants or to purify plasmid DNA suitable for sequencing
Sephadex G-50 spun column purificati
volume, to remove protein contaminants or to purify plasmid DNA suitable for sequencing. 1) Plug a 1ml syringe barrel with siliconized glass wool and fill to the top with a sterile suspension of Sephadex G50 equilibrated with H2 O. 2) Once filled place
Sephadex G-50 spun column purification
Sephadex G-50 spun column purification Spin column purification can be used to change buffers without a concomitant change in solution volume, to remove protein contaminants or to purify plasmid DNA suitable for sequencing. 1) Plug a 1
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