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NADH/NADPH荧光成像试剂盒 *深红色荧光*

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  • ¥7178
  • AATBIO
  • 15295
  • 2025年12月12日
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    上海研卉生物科技有限公司
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    • 详细信息
    • 询价记录
    • 技术资料
    • 英文名：

      Cell Meter™ Intracellular NADH/NADPH Fluorescence Imaging Kit *Deep Red Fluorescence*

    • 供应商：

      上海研卉生物科技有限公司

    • 库存：

      3W

    • 规格：

      100 Tests

    NADH/NADPH荧光成像试剂盒 *深红色荧光*

    细胞Meter™ 胞内烟酰胺腺嘌呤二核苷酸/烟酰胺腺嘌呤二核苷酸磷酸荧光成像试剂盒 *深红色荧光*
    英文名: Cell Meter™ Intracellular NADH/NADPH Fluorescence Imaging Kit *Deep Red Fluorescence*

    细胞内二氢烟酰胺腺嘌呤二核苷酸NADH及其磷酸酯NADPH的检测对疾病诊断和药物开发具有重要意义。总的来说，氧化还原偶联NAD/NADH和NADP/NADPH在能量代谢、糖酵解、三羧酸循环和线粒体呼吸中起着关键作用。细胞内NAD（P）H水平的升高与活性氧（ROS）的异常产生和DNA损伤有关。然而，由于缺乏敏感的NAD（P）H探针，在生物系统中检测细胞内NAD（P）H一直是一个挑战Cell Meter™细胞内NADH/NADPH荧光成像试剂盒提供了一种在远光谱中监测活细胞内NAD（P）H水平的有效方法，可与其他应用相结合，如GFP表达细胞或应用有丝分裂跟踪器。JJ1902 NAD（P）H传感器是一种用于检测和成像细胞内NADH/NADPH的荧光探针该探针具有荧光性质，能与NADH/NADPH结合，产生高灵敏度、高特异性的强荧光信号JJ1902 NAD（P）H传感器可以很容易地加载到活细胞中，其荧光信号可以使用Cy5®滤波器组方便地监测该试剂盒针对荧光成像和微孔板阅读器应用进行了优化。
    Protocol summary
    1. Prepare cells in growth medium
    2. Incubate cells with test compounds and JJ1902 NAD(P)H Sensor working solution at 37 oC 20 - 30 minutes
    3. Wash and keep cells in Assay Buffer
    4. Monitor fluorescence intensity (bottom read mode) at Ex/Em = 590/655 nm (Cutoff = 610 nm) or fluorescence microscope with Cy5® filter
    Important
    Thaw all the kit components at room temperature before starting the experiment.
    Key parameters
    Instrument: Fluorescence microscope
    Excitation: 590 nm
    Emission: 655 nm
    Recommended plate: Black wall/clear bottom
    Instrument specification(s): Cy5 filter
    Instrument: Fluorescence microplate reader
    Excitation: 590 nm
    Emission: 655 nm
    Cutoff: 610 nm
    Recommended plate: Black wall/clear bottom
    Instrument specification(s): bottom read mode
    Preparation of working solution
    Add 10 µL of JJ1902 NAD(P)H Sensor stock solution (Component A) into 2.5 mL of Assay Buffer (Component B), and mix well. This JZL1707 NAD(P)H Sensor working solution is stable within 1 hour at room temperature. Note: 40 µL of JJ1902 NAD(P)H Sensor stock solution is enough for one plate. Protect from light.
    For guidelines on cell sample preparation, please visit
    https://www.aatbio.com/resources/guides/cell-sample-preparation.html
    Sample experimental protocol
    1. To stimulate NADP/NADPH, treat cells with 10 µL of 10X test compounds (96-well plate) or 5 µL of 5X test compounds (384-well plate) in serum free medium or your desired buffer (such as PBS or HHBS). For control wells (untreated cells), add the corresponding amount of medium or compound buffer. Note: JJ1902 NAD(P)H sensor is compatible in the presence of serum as well. The optimization of the conditions for the sensor is highly recommended cell line to cell line.
    2. Add 100 µL/well (96-well plate) or 25µL/well (384-well plate) of JJ1902 NAD(P)H Sensor working solution in the cell plate. Co-incubate cells with test compound and JJ1902 NAD(P)H Sensor working solution at 37 oC for 20-30 minutes, protected from light. Note: For a NADH/NADPH positive control treatment: HeLa cells were incubated with 100 µM NADH or NADPH for 30 minutes in serum-free medium, and co-incubated with JJ1902 NAD(P)H sensor working solution at 37 oC for another 30 minutes. See Figure 1 for details.
    3. Wash cells with your desired buffer once. Remove solution in each well and add Assay Buffer (Component B) 100 µL/well for a 96-well plate or 25 µL/well for a 384-well plate.
    4. Monitor the fluorescence increase using microplate reader at Ex/Em = 590/655 nm (Cutoff = 610 nm) with bottom read mode, OR take images using fluorescence microscope with the filter set of Cy5® filter.
    Example data analysis and figures
    产品细节图片1
    Figure 1. Fluorescence images of NADH/NADPH in HeLa cells using Cell Meter™ Intracellular NADH/NADPH Fluorescence Imaging Kit (Cat#15295). HeLa cells were incubated with 100 µM NADH or 100 µM NADPH in serum-free medium for 30 minutes and then co-incubated with JJ1902 NAD(P)H sensor working solution for another 30 minutes. The fluorescence signal was measured using fluorescence microscope with a Cy5® filter.
    Disclaimer
    AAT Bioquest provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please consult the Safety Data Sheet (SDS) provided for the product. Chemical analysis and/or reverse engineering of any kit or its components is strictly prohibited without written permission from AAT Bioquest. Please call 408-733-1055 or email info@aatbio.com if you have any questions.


    Interactive Product Finder

    Product Ordering Information for NADP/NADPH Assay Kits

    Cat# Product Name Ex (nm) Em (nm) Size Price
    15260 Amplite™ Colorimetric Total NADP and NADPH Assay Kit 575 N/A 400 Tests $295
    15276 Amplite™ Colorimetric Total NADP and NADPH Assay Kit *Enhanced Sensitivity* 460 N/A 400 Tests $295
    15274 Amplite™ Colorimetric NADP/NADPH Ratio Assay Kit 460 N/A 250 Tests $345
    15272 Amplite™ Colorimetric NADPH Assay Kit 460 N/A 400 Tests $245
    15295 Cell Meter™ Intracellular NADH/NADPH Fluorescence Imaging Kit *Deep Red Fluorescence* 593 655 100 Tests $495
    15281 Amplite™ Fluorimetric NADP Assay Kit *Blue Fluorescence* 422 466 200 Tests $345
    15264 Amplite™ Fluorimetric NADP/NADPH Ratio Assay Kit *Red Fluorescence* 571 585 250 Tests $345
    15262 Amplite™ Fluorimetric NADPH Assay Kit *Red Fluorescence* 571 585 400 Tests $295
    15266 ReadiUse™ NADP Regenerating Kit N/A N/A 200 Tests $245
    15265 ReadiUse™ NADPH Regenerating Kit N/A N/A 1000 Tests $295
    Alternative forms
    Product name Current Product Related Product
    Cell Meter™ Intracellular NADH/NADPH Fluorescence Imaging Kit
    Deep Red Fluorescence

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