Collagen functions as a structural component of the body, providing strength and support to the extracellular matrix (ECM), and has also been shown to be highly involved in cell signaling. The physical properties of collagen are well-suited for biomedical research such as 3D cell culture, as well as regenerative medicine and medical devices.
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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
3mg/mL Pepsin Soluble Collagen in 0.01M HCI 100ml
- 供应商:
上海研卉生物科技有限公司
- 库存:
1
- 规格:
100 ml
3mg/mL Pepsin Soluble Collagen in 0.01M HCI 100ml
Collagen Formulations
| Pepsin Solubilized Atelocollagen, 3mg/ml or 6 mg/ml |
Highly purified bovine corium Type I pepsin solubilized collagen, atelocollagen, in 0.01 N HCl. Forms a gel when at pH 7 and is ideal for 3D scaffolds for culture or coating plastic ware for increased cell adhesion. |
|
| Acid Solubilized Telo Collagen, 6mg/ml |
Highly purified bovine corium Type I acid solubilized collagen, telocollagen, in 0.01 N HCl. Forms a gel when at pH 7 and is ideal for 3D scaffolds for culture or coating plastic ware for increased cell adhesion. |
|
| Titration Buffer | Titration buffer (0.2 phosphate, pH11.2) Premixed titration buffer to be used with pepsin or acid solubilized collagen for forming 3D gels |
|
Fibrillar Collagen |
Fibrillar Collagen, 65 mg/ml | Highly purified bovine corium Type I provided at pH 7. Ideal for cell and drug delivery and wound applications. |
|---|---|---|
Freeze Dried Collagen |
Standard Lyophilized Collagen |
Highly purified bovine tendon Type I polymeric collagen in the native state (telocollagen). Provided in sheet or milled form. Ideal for further processing to fabricate custom formulations. |
| Long Fiber Lyophilized Collagen |
Highly purified bovine corium Type I acid solubilized collagen, telocollagen, in 0.01 N HCl. Forms a gel when at pH 7 and is ideal for 3D scaffolds for culture or coating plastic ware for increased cell adhesion. |
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文献和实验the pellet in 10 ml sterile 1 X TE/LiAC (made fresh from stocks of 10 X TE [0.1 M Tris-HCI, 0.01 M EDTA, pH 7.5] and 10X LiAC [1 M lithium acetate adjusted to pH 7.5 with dilute acetic acid]. Repeat. Pellet cells in microfuge at 7000rpm, discard sup
Bromodeoxyuridine Immunohistochemistry
endogenous peroxidases in a staining dish. 5. 1XPBS, 4 X 5 min., RT 6. Pepsin/HCI, 20 min. (try 30 - 40 min.), RT in a humidifies chamber. Prepare 200 mg/ml pepsin, 0.01N HCI in PBS [from 10 mg/ml pepsin prepared fresh in ddH2O(50x) and 2N HCI (200
消化液 1、0.1%胰蛋白酶 试剂:胰蛋白酶 0.1gm 0.1%氯化钙(pH7.8) 100ml 配制方法:先配制0.1%的CaCl2,用0.1mol/L的NaOH将其pH调至7.8,然后加入蛋白酶溶解之。用前将胰蛋白酶消化液在水浴中予热至37℃(载有标本的玻片也在TBS中予热至同样样温度)。该消化液时间约为5~30min。 2、0.4%胃蛋白酶 试剂:胃蛋白酶 400mg 0.1N HCl 100ml 配制方法:同胰蛋白
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