<20.0 EU per 1 μg of the protein by the LAL method.
Activity
Applications:
Substrate for cell culture adhesion. An area of 25 cm2 can be coated with 0.5 ml of a 0.01% Poly-L-Lysine solution. Optimal conditions for attachment must be determined for each cell line and application. Slides may be dipped in the solution and air dried before applying sample.
Quality Control:
Tested for ability to promote attachment of rat PC-12 pheochromocytoma cells.
Cell Culture Qualified: No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP XXIV, Chapter 71 sterility testing. No mycoplasma contamination detected by PCR.
Source
Fermentation using starch.
Background: Poly-L-Lysine
This highly positively charged amino acid chain is commonly used as a coating agent to promote cell adhesion in culture. This solution is provided ready to use at 0.01% and contains polymers in the 70,000–150,000 kDa range.