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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Foxp3转录因子染色缓冲液
- 供应商:
上海研卉生物科技有限公司
- 抗体英文名:
eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set
- 是否单克隆:
多克隆
- 抗原来源:
Goat,Rabbit,Mouse
- 保质期:
一年
- 库存:
大量
- 保存条件:
低温
- 规格:
1 kit
Foxp3转录因子染色缓冲液
描述
Foxp3/Transcription Factor Staining Buffer Set 专利配方设计和优化,可使用抗体对转录因子和核蛋白(例如 Foxp3 和 Ki-67)以及细胞因子和趋化因子进行核内染色。规格
| Prop-65 Warning: | Control Code 65 |
|---|---|
| Product Line: | eBioscience™ |
| Product Size: | 1 kit |
| Reagent Type: | Fixation Diluent, Fixation Reagent, Permeabilization Diluent, Permeabilization Reagent |
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文献和实验A general protocol for staining cell for cytometry analysis
General Annexin V Staining Procedure Solutions 1.10X Binding Buffer (Cat. No. 66121A):0.1 M HEPES, pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Dilute to 1× prior to use. 2. Propidium Iodide (PI). Prepare a 50 µg/ml stock solution of PI in 1× PBS buffer
Intracellular Immunofluorescent Staining for Flow Cytometry
ligand. Buffers used for intracellular staining can have varying effects. eBioscience antibodies are optimized using eBioscience Foxp3 staining buffer set (cat. 00-5523 ) or eBioscience IC Fixation Buffer (cat. 00-8222 ). All antibodies to cytokines
A general protocol for staining cell for cytometry analysis
Staining 1. Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of ~1 x 10 6 cells/ml. 2. Transfer 100 µl of the solution (~1 x 10 5 cells) to a 5 ml culture
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