- ¥3062
- BD
- 561011/550821
- USA
- 2025年10月03日
- IHC/WB/ELISA
- Rabbit
- 人，小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 适应物种：
人，小鼠
- 应用范围：
IHC/WB/ELISA
- 库存：
大量
- 抗体英文名：
PE Rabbit Anti- Active Caspase-3
- 是否单克隆：
多克隆
- 宿主：
Rabbit
- 保存条件：
低温
- 供应商：
上海研卉生物科技有限公司
- 抗原来源：
Goat，Rabbit，Mouse
- 保质期：
一年
- 规格：
25T
BD Pharmingen
Alternative Name
CPP32; Yama; Apopain
Vol. Per Test
20 µl
Isotype
Rabbit IgG
Reactivity
Human (QC Testing) Mouse (Tested in Development)
Application
Intracellular staining (flow cytometry) (Routinely Tested)
Immunogen
Human Active Caspase-3 Fragment
Description
The caspase family of cysteine proteases plays a key role in apoptosis and inflammation. Caspase-3 is a key protease that is activated during the early stages of apoptosis and, like other members of the caspase family, is synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another protease. The processed forms of caspases consist of large (17-22 kDa) and small (10-12 kDa) subunits which associate to form an active enzyme. Active caspase-3, a marker for cells undergoing apoptosis, consists of a heterodimer of 17 and 12 kDa subunits which is derived from the 32 kDa pro-enzyme. Active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cytoplasm, e.g., D4-GDI and Bcl-2, and in the nucleus (e.g. PARP). This antibody has been reported to specifically recognize the active form of caspase-3 in human and mouse cells. It has not been reported to recognize the pro-enzyme form of caspase-3.
Format
- Format
- PE
- Excitation Source
- Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max
- 496 nm
- Emission Max
- 578 nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
Suggested Companion Products
| Cat No. | Description | Size | |
|---|---|---|---|
| 554714 | Fixation/Permeabilization Solution Kit RUO |
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
干货 | CUT and Tag 让 ChIP-Seq更简单高效！
说明书推荐的反应体系进行 CUT&Tag 实验；其中，pG-Tn5 转座子使用的终浓度为 0.04μM ，一抗为 H3K27me3（CST，#9733），二抗为 Goat anti Rabbit（Bioworld，#BS13271）。 A、文库峰型检测 使用 Agilent 2100 Bioanalyzer 进行文库分布检测。 图 2. 使用 TD901 构建 100 cells CUT&Tag 文库峰型图 图 3. 使用 TD901 构建 10,000 cells CUT&Tag
Protocol for intracytoplasmic staining of cytokines for FACS analysis
; Pharmingen 18195A) for FL2 anti-IL10 (JES5.16E3-PE; Pharmingen 18435A) for FL1 anti-TGF-beta (rabbit anti pan TGF-beta; R&D Systems AB-100-NA home made FITC conjugate) for FL2 8) Wash extensively in saponin buffer followed by PBS + 0.1% azide
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