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- 详细信息
- 文献和实验
- 技术资料
- 库存:
121
- 英文名:
Recombinant Human Epithelial Cell Adhesion Molecule
- 保质期:
长期
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
冻干蛋白置于-20℃以下可长期保存,室温
- 规格:
10μg|50μg|500μg|1mg
特别提示:包括重组人上皮细胞粘附分子(Ep-CAM)在内,本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
产品名称:重组人上皮细胞粘附分子(Ep-CAM)
英文名称:Recombinant Human Epithelial Cell Adhesion Molecule
产品货号:JN0521
产品规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的Gln24-Lys265在C端含有His标签。
Ep-CAM质量控制:>95%(还原性SDS-PAGE)
Ep-CAM制剂:冻干品
Ep-CAM保存:
冻干蛋白置于-20℃以下可长期保存,室温条件下可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天,可分装后置于-20℃保存三个月。
Ep-CAM复溶:
打开试剂管前请先离心。
复溶浓度推荐大于100 μg/ml。
冻干蛋白请溶于ddH2O。
复溶后,请根据用量分装冻存,避免反复冻融。
关于Ep-CAM:
Epithelial Cell Adhesion Molecule (EpCAM) is a signal type I transmembrane glycoprotein that belongs to the EPCAM family. EpCAM is composed of an extracellular domain with one thyroglobulin type-1 domain, a transmembrane domain and a cytoplasmic domain. EpCAM is found on the surface of adenocarcinoma, but not on mesodermal or neural cell membranes. The EpCAM molecule has been shown to function as a homophilic Ca2+ independent adhesion molecule. It may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium as an immunological barrier providing the first line of defense against infection. Defects in EPCAM are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) and diarrhea type 5 (DIAR5). EpCAM plays a role in embryonic stem cells proliferation and differentiation; it up-regulates the expression of FABP5, MYC and Cyclin A and Cyclin E. It is highly and selectively expressed by undifferentiated embryonic stem cells.
除了重组人上皮细胞粘附分子(Ep-CAM),,我公司还供应以下相关产品:
| 货号 | 名称 | 规格 |
| JN0453 | 重组人TNFRSF6(CD95) | 10μg|50μg|500μg|1mg |
| JN1922 | 重组人细胞珠蛋白(Cytoglobin) | 10μg|50μg|500μg|1mg |
| JN1930 | 重组人脂肪酸结合蛋白2(FABP2) | 10μg|50μg|500μg|1mg |
| JN0539 | 重组人PDGFR-β(血小板衍生生长因子受体β) | 10μg|50μg|500μg|1mg |
| JN0663 | 重组人分泌型卷曲相关蛋白2(sFRP-2) | 10μg|50μg|500μg|1mg |
| JN0799 | 重组人LYG2(类球蛋白G蛋白2) | 10μg|50μg|500μg|1mg |
| JN1088 | 重组人Jagged-1蛋白(JAG1) | 10μg|50μg|500μg|1mg |
| JN1137 | 重组人聚集素(Clusterin) | 10μg|50μg|500μg|1mg |
| JN1139 | 重组人钙粘蛋白-6(CDH6) | 10μg|50μg|500μg|1mg |
| JN1219 | 重组人神经胶质成熟因子-β(GMF-β) | 10μg|50μg|500μg|1mg |
| JN1396 | 重组小鼠IL-1RN | 10μg|50μg|500μg|1mg |
| JN1407 | 重组人BMP9(GDF2) | 10μg|50μg|500μg|1mg |
| JN1480 | 重组人促卵泡激素(FSH) | 10μg|50μg|500μg|1mg |
| JN1688 | 重组人ACPP(前列腺酸性磷酸酶) | 10μg|50μg|500μg|1mg |
| JN1725 | 重组人HAI2(肝细胞生长因子激活因子抑制剂-2) | 10μg|50μg|500μg|1mg |
| ZN1555 | 人Angiogenin重组蛋白 | 100ng |
| ZN1675 | 人MIF重组蛋白 | 100ng |
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文献和实验8 eggs per day, day 7- day 13 cut CAM, wash in precooled PBS, in 10 ml WASH 1 (PBS, 5 mM EDTA, COMPLETE) on ice cut in pieces in petri dish on ice centrifuge 4 min at 1000 rpm (table top centifuge, Falcon 15 ml tubes) at 4°C
白细胞和血管内皮细胞、巨噬细胞和上皮细胞等以及血液中均存在细胞粘附分子。按其结构特1瀀,分有钙离子依赖性家族、整合素家族、免疫球蛋白超家族和选择素家族四大类。在各种细胞因子、内毒素、凝血酶等作用下,细胞粘附分子由细胞内贮存池转移至细胞膜或合成增加,导致细胞表面的粘附分子数量增多。因此在某些疾病中细胞粘附分子的数量有改变。此外,细胞粘附分子经磷酸化、糖基化等修饰作用可发生构象改变,导致其亲和力和扩散速率改变而影响其功能,因此定量检测细胞粘附分子的数量
The potential irritancy of compounds may be detected by observing adverse changes that occur in the chorionallantoic membrane of the egg after exposure to test chemicals (1 ).
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