Mouse/Human/Rat Phospho-BAD (S
er136) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1459- $740
- LSBio
- 详见官方网站
- LS-F1459
- 美国
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Mouse, Human, Rat
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
详见官方网站
- 供应商：
康朗生物
- 规格：
96T
Performance 100% Guaranteed
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Product Description
LS-F1459 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human BAD in samples of Adherent Cell Cultures. It is based upon a Cell-Based Phosphorylation assay principle.About BAD
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain containing pro-apoptotic proteins, such as Bax, Bid, and Bik, form a growing subclass of the Bcl-2 family. Another such protein is the Bcl-2-antagonist of cell death (Bad). Bad regulates apoptosis by forming heterodimers with anti-apoptotic proteins Bcl-2 and Bcl-xL, thereby preventing them from binding with Bax. Q92934 NM_004322 NP_004313.1
BAD ElisaKit, BBC2 ElisaKit, Bcl-2-like protein 8 ElisaKit, BCL2-binding component 6 ElisaKit, BCL2L8 ElisaKit, BBC6 ElisaKit, BCL-X/BCL-2 binding protein ElisaKit, Bcl-2-binding component 6 ElisaKit, Bcl2 antagonist of cell death ElisaKit, BCL2-binding protein ElisaKit, Bcl2-L-8 ElisaKit
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Specifications
TypeCell-Based Phoshorylation ELISA (enzyme-linked immunosorbent assay) kit
Target
BAD
Reactivity
Mouse, Human, Rat
Specificity
BAD (Phospho-Ser136)
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- 96-Well Cell Culture Clear-Bottom Microplate
- 10x TBS
- Quenching Buffer
- Blocking Buffer
- 10x Wash Buffer
- 100x Anti-BAD (Phospho-Ser136) Antibody (Rabbit Polyclonal)
- 100x Anti-BAD Antibody (Rabbit Polyclonal)
- 100x Anti-GAPDH Antibody (Mouse Monoclonal)
- HRP-Conjugated Anti-Rabbit IgG Antibody
- HRP-Conjugated Anti-Mouse IgG Antibody
- Primary Antibody Diluent
- Ready-to-Use Substrate
- Stop Solution
- Crystal Violet Solution
- SDS Solution
- Adhesive Plate Seals
Protocol
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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Cell-Based Phosphorylation ELISA Platform Overview
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
in many signaling pathways typically associated with human malignancies. Phospho?motif antibodies are engineered to recognize proteins that contain a phosphorylated residue in the context of a specific motif. They are raised against a library of phospho?peptides
=result&resnum=1#v=onepage&q=gsk%20tyrosine%20phosphorylation%20and%20activity&f=false By the way, based on my own experience, most of antibodies from Santa Cruz are NOT reliable at all. I strongly recommend Cell Signaling Technology (CST
TBS 和 5% NGS 中封闭样品。市售的含有酪蛋白的封闭溶液与磷酸化的一抗结合后易减弱信号；因此，我们建议不使用含有酪蛋白的封闭剂进行磷酸化特异性抗体的检测。以上针对封闭步骤所提建议均是在以 SignalStain. Boost IHC Detection Reagent 作为检测试剂的情况下提出的。如果选择使用其他检测试剂，我们则建议使用与二抗来源相同的血清进行封闭。抗体：Phospho–Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718样本：石蜡
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