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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-9984R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
C22orf25
- 抗体名:
22号染色体开放阅读框25抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Zebrafish,
- 免疫原:
KLH conjugated synthetic peptide derived from human C22orf25:21-120/276
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | C22orf25 |
| 中文名称 | 22号染色体开放阅读框25抗体 |
| 别 名 | Uncharacterized protein C22orf25; chromosome 22 open reading frame 25; DKFZp 761 P 1121; Hypothetical protein LOC128989; TNG2_HUMAN. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Zebrafish, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 31kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human C22orf25:21-120/276 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Chromosome 22 contains over 500 genes and about 49 million bases. Being the second smallest human chromosome, 22 contains a surprising variety of interesting genes. Phelan-McDermid syndrome, Neurofibromatosis type 2 and autism are associated with chromosome 22. A schizophrenia susceptibility locus has been identified on chromosome 22 and studies show that 22q11 deletion symptoms include a high incidence of schizophrenia. Translocations between chromosomes 9 and 22 may lead to the formation of the Philadelphia Chromosome and the subsequent production of the novel fusion protein, BCR-Abl, a potent cell proliferation activator found in several types of leukemia. The C22orf25 gene product has been provisionally designated C22orf25 pending further characterization. SWISS: Q6ICL3 Gene ID: 128989 Database links: Entrez Gene: 128989 Human SwissProt: Q6ICL3 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Sample:Lovo Cell (Human) Lysate at 30 ug Primary: Anti- C22orf25 (bs-9984R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31kD Observed band size: 35kD ![]() Sample:Placenta (Mouse) Lysate at 40 ug Primary: Anti- C22orf25 (bs-9984R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31kD Observed band size: 35kD ![]() Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C22orf25) Polyclonal Antibody, Unconjugated (bs-9984R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. |
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文献和实验,然而这还仅仅只是一个假说;1960年Nowell和Hungerford在7例慢性髓系白血病(chronic myeloid leukemia,CML)的患者中发现后来被称为费城染色体(Philadelphia chromosome)的微小染色体;1973年Rowley证实了Ph染色体是9号和22号染色体易位所致,这是人们在肿瘤中认识到的第一个染色体易位;目前,已经有11,500篇文献报道了55,600多种克隆性细胞遗传学异常。这些染色体畸变,尤其是染色体易位及其相应的融合基因在肿瘤致病的起始阶段有着
长度为1450bp即483个密码子,最长的是位于XII号染色体上的一个功能未知的开放阅读框(4910个密码子),还有极少数的开放阅读框长度超过1500个密码子。在酵母基因组中,也有编码短蛋白的基因,例如,编码由40个氨基酸组成的细胞质 膜蛋白 脂质的PMP1基因。此外,酵母基因组中还包含:约140个编码RNA的基因,排列在XII号染色体的长末端;40个编码SnRNA的基因,散布于16条染色体;属于43个家族的275个tRNA基因也广泛分布于基因组中。 表1 酵母染色体
,即整个基因组有72%的核苷酸顺序由开放阅读框组成。这说明酿酒酵母基因要比其它高等真核生物基因排列紧密。如在人类基因组中,大约平均每隔30kb或更多的碱基才能发现一个编码蛋白质的基因。酵母基因组的紧密性是因为基因间隔区较短与基因中内含子稀少。酵母基因组的开放阅读框平均长度为483个密码子,最长的是位于XII号染色体上的一个功能未知的开放阅读框(4910个密码子)。此外,酿酒酵母基因组中还包含有大约140个编码rRNA的基因,排列在XII号染色体的长末端;40个编码snRNA(small nuclear
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