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- SignalChem
- 加拿大
- M42-51N-BULK
- 2025年07月16日
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- 供应商：
安诺伦（北京）生物科技有限公司
存储温度：Store product at –20oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
存储溶液：100mM MOPS, pH 6.5, 0.25mM DTT and 0.1mM PMSF.
参考文献：1. Chevalier D, et al. Purification of myelin basic protein from bovine brain. Protein Expr Purif. 18(2):229-34, 2000. 2. Sheng HZ, et al. Developmental study of myelin basic protein variants in various regions of pig nervous system. J Neurochem. 1989 Mar;52(3):736-40. 3. Sanghera J. et al. Identification of the sites in myelin basic protein that are phosphorylated by meiosis-activated protein kinase p44mpk. FEBS Lett. 1990 Oct 29;273(1-2):223-6. 4. Martenson, et al., Identification of multiple in vivo phosphorylation sites in rabbit myelin basic protein. J. Biol. Chem. 258: 930, 1983.
应用：Kinase Assay, Western Blot
官网链接：https://www.signalchem.com/product_details.php?id=1348
商品关键词：MBP Protein,M42-51N-BULK,SignalChem
简单描述：Native Swine MBP was extracted under acidic conditions and further purified by cation chromatography from pig brain (1). This product is routinely evaluated using active MAP Kinase 3/ERK1.
Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet. Molecular Weight: ~21.5 kDa Scientific Background: MBP exists as four major forms in the pig CNS with apparent molecular weights of 21.5K, 20.2K, 18.5K, and 17.3K (2). Native pig MBP is extracted under acidic conditions and further purified by cation chromatography. MBP is an efficient substrate for numerous protein kinases including ERK/MAP Kinase family, cAMP-dependent Protein Kinase, Calmodulin-dependent Protein Kinase, Protein Kinase C, and Phosphorylase Kinase (3, 4).
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文献和实验Factor Xa Cleavage of MBP-Fusion protein
INTRODUCTION In many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix. The amount of factor Xa, temperature and length of incubation must be calibrated
Protein Expression and Purification of MBP-T antigen (131-259)
Comments: T antigen (131-259)is an MBP-Hisx6-fusion protein constructed by Laura Mizoue (refer to the wisdom page for details on the construct). i.Pick a single colony from freshly transformed cells (BL21 (DE3)is the expression host)into 2 ml of LB
LuMPIS: Luciferase-Based MBP-Pull-Down Protein Interaction Screening System
Analyzing the putative interaction partners of an individual protein is one approach to elucidate its function. In the LuMPIS protocol, bait and prey proteins are expressed with N-terminal maltose binding protein (MBP)- and eGFP-luciferase
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