- ￥16908
- SignalChem
- 加拿大
- S07-911-20
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
安诺伦（北京）生物科技有限公司
- 规格：
20 nmol
存储温度：The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.
存储溶液：The siRNAs are supplied as a lyophilized powder and shipped at room temperature.
参考文献：1. Kobayashii, T. et al: Characterization of the structure and regulation of two novel isoforms of serum- and glucocorticoid-induced protein kinase. Biochem J. 1999 Nov 15;344 Pt 1:189-97. 2. Boehmer, C. et al: Stimulation of renal Na+ dicarboxylate cotransporter 1 by Na+/H+ exchanger regulating factor 2, serum and glucocorticoid inducible kinase isoforms, and protein kinase B. Biochem Biophys Res Commun. 2004 Jan 23;313(4):998-1003.
官网链接：https://www.signalchem.com/product_details.php?id=7938
商品关键词：SGK2 siRNA Set I,,S07-911-20,SignalChem
简单描述：SGK2 is a pool of three individual synthetic siRNA duplexes designed to knock-down human SGK2 mRNA expression. Each siRNA is 19-25 bases in length.
Description: SGK2 is a pool of three individual synthetic siRNA duplexes designed to knock-down human SGK2 mRNA expression. Each siRNA is 19-25 bases in length.
Reconstitution Protocol: Briefly centrifuge the tubes (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend the siRNA in 50 ul of DEPC-treated water (supplied by researcher), which results in a 1x stock solution (10 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Format: Lyophilized powder
Gene Aliases: H-SGK2; dJ138B7.2
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文献和实验Our current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western blotting. This allows us to test several candidate siRNAs quite cheaply. The yields are good enough for reasonable
engine performs a search for off-target hits using the set of obtained siRNA candidate sequences as the query against the refseq RNA database. There are two advantages over most BLAST servers. First, most BLAST servers use the entire nr nucleic sequences
starter kits that include a set of controls for the siRNA transfection and Western blot analysis. Transfection of siRNA duplexes We perform a single transfection of siRNA duplex using OLIGOFECTAMINE Reagent (product number: 12252011
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