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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-9450R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
5 MethylCytosine
- 抗体名:
5甲基胞嘧啶抗体
- 宿主:
Rabbit
- 适应物种:
5 MethylCytosine
- 免疫原:
KLH conjugated 5 MethylCytosine:
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
ELISA=1:500-1000 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul
5 MethylCytosine 5甲基胞嘧啶抗体
| 英文名称 | 5 MethylCytosine |
| 中文名称 | 5甲基胞嘧啶抗体 |
| 别 名 | 5-Methyl Cytidine; 5 m C; 5 mC; 5 me C; 5 Me Cytidine; 5 MeCyd; 5 Methyl Cytidine; 5 MethylCytosine; 5-mC; 5-Me Cytidine; 5-Methyl-Cytosine; 5-MethylCytidine. |
| 规格价格 | 100ul/1580元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 药物与化合物抗体 |
| 研究领域 | 细胞生物 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | 5 MethylCytosine |
| 产品应用 | ELISA=1:500-1000 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 0.16159kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated 5 MethylCytosine: |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20癈. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 癈. |
| PubMed | PubMed |
| 产品介绍 | background: Cytidine is a nucleoside formed by a cytosine attached to a ribose ring via a beta-N1-glycosidic bond. DNA is methylated on cytidines by DNA methylases (DNMTs)to generate 5-methylcytidine (5-mC), a potent epigenetics marker and regulator of gene expression. The reverse reaction (cytidine demethylation) starts with its oxidation to hydroxymethyl- (5-hmC), formyl- (5-fC), and carboxy- (5-caC) cytidine. Several enzymes, including the Tet family of proteins have been implicated in cytidine demethylation. Subcellular Location: Nuclear. SWISS: N/A CAS: 58366-64-6 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验相关实验
许多动、植物 DNA的脱氧核苷酸的嘧啶碱基之一。例如在小牛胸腺核酸和麦胚芽的 DNA中有大量存在。在动、植物的 RNA中也含有微量。其生物学意义过去很长时期欠明,但近年已明确它在控制基因表达方面起重要作用。
。 4.DNA拓扑结构变化 天然双链DNA的构象大多是负性超螺旋。当基因活跃转录时,RNA聚合酶转录方向前方DNA的构象是正性超螺旋,其后面的DNA为负性超螺旋。正性超螺旋会拆散核小体,有利于RNA聚合酶向前移动转录,而负性超螺旋则有利于核小体的再形成。 5.DNA碱基修饰变化 真核DNA中的胞嘧啶约有5%被甲基化为5―甲基胞嘧啶(5―methylcytosine,m5C),而活跃转录的DNA片段中胞嘧啶甲基化程度常
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5 MethylCytosine 5甲基胞嘧啶抗体
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