WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
49kDa
细胞定位
细胞核 细胞浆
性 状
Lyophilized or Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthetic peptide derived from human Proteasome 19S S7:51-150/433
亚 型
IgG
纯化方法
affinity purified by Protein A
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed
PubMed
产品介绍
background: The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. In case of HIV-1 infection, positive modulator of Tat-mediated transactivation.
Function: The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. In case of HIV-1 infection, positive modulator of Tat-mediated transactivation.
Subunit: Interacts with NDC80 and SQSTM1. Interacts with PAAF1. Interacts with HIV-1 Tat. Interacts with TRIM5.
Subcellular Location: Cytoplasm. Nucleus. Cytoplasm, P-body (By similarity). Note=Co-localizes with TRIM5 in the cytoplasmic bodies (By similarity).
Similarity: Belongs to the AAA ATPase family.
SWISS: P35998
Gene ID: 5701
Database links:
Entrez Gene: 5701 Human
Entrez Gene: 19181 Mouse
Entrez Gene: 25581 Rat
Omim: 154365 Human
SwissProt: P35998 Human
SwissProt: P46471 Mouse
SwissProt: Q63347 Rat
Unigene: 437366 Human
Unigene: 2462 Mouse
Unigene: 1202 Rat
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片
Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-PSMC2/Proteasome 19S S7 (bs-9349R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 50 kD
Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-PSMC2/Proteasome 19S S7 (bs-9349R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 52 kD