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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-9183R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
VGLL2
- 抗体名:
转录辅助因子退变样蛋白2抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
- 免疫原:
KLH conjugated synthetic peptide derived from human VGLL2:101-200/317
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | VGLL2 |
| 中文名称 | 转录辅助因子退变样蛋白2抗体 |
| 别 名 | Protein VITO1; Transcription cofactor vestigial like 2; Transcription cofactor vestigial like protein 2; Transcription cofactor vestigial-like protein 2; Vestigial like 2; Vestigial like 2 (Drosophila); Vgl-2; VGL2; VGLL 2; Vgll2; VGLL2_HUMAN; VITO1. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 干细胞 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 33kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human VGLL2:101-200/317 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Vgl-2, also known as VITO-1, is a 317 amino acid protein that contains a domain through which it interacts with TEF-1, a protein that plays a role in controlling the expression of numerous genes. Specific to skeletal muscle, Vgl-2 is expressed highly in adult fast muscle and is expressed at lower levels in adult slow muscle and fetal skeletal muscle. During muscle differentiation, Vgl-2 mRNA levels increase and Vgl-2 translocates from the cytoplasm to the nucleus. Overexpression of Vgl-2 in MYOD-transfected 10T1/2 mouse embryonic fibroblasts increases expression of myosin heavy chain (MHC), which is a marker of terminal muscle differentiation. This evidence suggests that Vgl-2 is essential for muscle gene expression. There are two isoforms of Vgl-2 that are produced as a result of alternative splicing events. Function: May act as a specific coactivator for the mammalian TEFs. May play a role in the development of skeletal muscles. Subunit: Interacts with TEFs. Binds to TEAD1/TEF1. Subcellular Location: Nucleus. Tissue Specificity: Skeletal muscle. Similarity: Belongs to the vestigial family. SWISS: Q8N8G2 Gene ID: 245806 Database links: Entrez Gene: 245806 Human Entrez Gene: 215031 Mouse Omim: 609979 Human SwissProt: Q8N8G2 Human SwissProt: Q8BGW8 Mouse Unigene: 99324 Human Unigene: 87237 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-VGLL2 (bs-9183R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 35 kD |
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文献和实验,从而将目的基因靶向性地导入肿瘤细胞。Khare等将抗人癌胚抗原(CEA)单链抗体编码基因与env基因重组,使反转录病毒将“自杀基因”――一氧化氮合成酶(NOS)靶向导人CEA阳性的肿瘤细胞,并通过过度合成NOS杀死肿瘤细胞。 靶向转移目的基因策略的工作原理 另一个例子是腺病毒。腺病毒载体在感染靶细胞时,首先依靠通过病毒衣壳(capsid)上的纤维蛋白(nber)头部的knob结构域识别并结合靶细胞表面的“柯萨奇病毒和腺病毒受体”(CAR),然后通过衣壳的五邻体蛋白
1 个关键药物,实现小鼠全能干细胞体外捕获和长期维持,北大杜鹏 Cell 报道新技术
背景介绍 干细胞研究与再生医学、辅助生殖、癌症、代谢紊乱和衰老有着广泛的联系,其中,在体外捕获和维持具有高分化潜能的胚胎干细胞 (ESCs) 是进行干细胞相关研究的基础。目前,体外培养的 ESCs 通常来自于内细胞团,能够分化为成年生物体的任何细胞类型,具有最高的发育潜能。但是到目前为止,在体外捕获和维持与体内全能卵裂球分子和功能相似的全能干细胞仍然是领域内的难题。 剪接体是由 5 个核心亚基和若干辅助因子组成的大分子核糖核蛋白复合体,是信使 RNA (mRNA) 剪接和成熟的动态分子机器
。非常适合用于鉴定转录辅助因子和染色质相关蛋白的相互作用。传统的鉴定蛋白相互作用的方法是 IP 之后进行质谱分析。与 IP 方法不同,RIME 使用的起始材料经过交联,主要检测的是染色质上蛋白之间的相互作用。 怎么检测同一基因组位置的两个蛋白质或不同的组蛋白修饰? 当进行 ChIP 实验去解码组蛋白编码时,证明两个不同的蛋白质或者组蛋白修饰占据同一个核小体位点是非常有意义的。或者,您也许需要确定一个蛋白质和特定的组蛋白修饰是否在同一个调控元件。
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