- ￥2625
- Biogems
- 美国
- 02512-25-50 ug
- 2025年07月14日
- FC, FA
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
The product should be stored undiluted at 4°C. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.
- 克隆性：
多克隆
- 供应商：
安诺伦（北京）生物科技有限公司
- 应用范围：
FC, FA
- 规格：
50 ug
来源：Armenian Hamster IgM, kappa
存储溶液：Phosphate-buffered aqueous solution, ph7.2.
参考文献：Kaneko, Y., Hirose, S., Abe, M., Yagita, H., Okumura, K., & Shirai, T. (1996). CD40‐mediated stimulation of B1 and B2 cells: implication in autoantibody production in murine lupus. European journal of immunology, 26(12), 3061-3065.Ferlin, W. G., Severinson, E., Ström, L., Heath, A. W., Coffman, R. L., Ferrick, D. A., & Howard, M. C. (1996). CD40 signaling induces interleukin‐4‐independent IgE switching in vivo. European journal of immunology, 26(12), 2911-2915.Kashiwada, M., Kaneko, Y., Yagita, H., Okumura, K., & Takemori, T. (1996). Activation of mitogen‐activated protein kinases via CD40 is distinct from that stimulated by surface IgM on B cells. European journal of immunology, 26(7), 1451-1458.
特异性：Mouse, Rat
官网链接：http://www.bio-gems.com/flow-cytometry/safire-functional-antibodies/anti-mouse-rat-cd40-safire.html
商品关键词：Anti-Mouse/Rat CD40 SAFIRE Purified,02512-25-50 ug,Biogems
简单描述：The HM40-3 monoclonal antibody reacts with mouse CD40, a 48kDA type I glycoprotein known as Bp50. CD40 is part of the TNFR family and expressed on a subset of T cells, B cells, dendritic cells, and macrophages. It has been shown to be involved in the protection of B cells from apoptosis, B cell differentiation, co-stimulation, and isotype class-switching. The HM40-3 antibody blocks the binding of CD40L (CD154) and stimulates B and dendritic cell proliferation.
Clone: HM40-3
Format: SAFIRE Purified
Cell Type: B Cells, Dendritic Cells, NK and NKT Cells, Monocytes and Macrophages
官网链接: www.annoron.com
办公电话: 010-56256916
官方售后: 400-965-8633
企业QQ: 3198592576、3212874516
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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
16 (Mouse BD Fc Block™; Cat No. 553141/553142) or anti-rat CD32 (Rat BD Fc Block™; Cat. No. 550270/550271) is recommended. BD Fc Block™ can be added to cells (~0.25 µg per million cells, 3 - 5 min, 4°C) and need not be washed out prior to addition of primary
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
Reproducible purification of DNA and RNA in 96-well format. Genomic DNA and total RNA were purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. A Purified DNA samples were analyzed on a 0.8% agarose gel. B
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