细胞色素C(Cyt-C)，anti-Cytochrome c

Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 6H2.B4 monoclonal antibody recognizes human, mouse, and rat cytochrome-c and has been shown to be useful for intracellular flow cytometric staining, Western blotting, immunoprecipitation, and immunofluorescence staining.

IP - Quality tested
ICC - Verified
ICFC - Reported in the literature, not verified in house

Each lot of this antibody is quality control tested by immunoprecipitation. This antibody can be used at 2.0 - 4.0 µg /1 x10⁷ cell equivalents for immunoprecipitation. For Western blot application, the suggested use of this reagent is 0.5 µg per ml (1:1000 dilution). For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional reported applications (for the relevant formats) include: intracellular flow cytometry⁵, immunofluorescence microscopy^3,5, immunoprecipitation⁴, and immunocytochemistry⁵.

Mitochondrial intermembrane space