Family with sequence similarity 13 member C1; family with sequence similarity 13, member C: Hypothetical protein LOC220965; MGC33233; RGD1310149; FA13C_HUMAN.
规格价格
100ul/1380元 购买 200ul/2200元 购买 大包装/询价
说 明 书
100ul 200ul
研究领域
肿瘤 细胞生物 免疫学
抗体来源
Rabbit
克隆类型
Polyclonal
交叉反应
Human, Mouse, Rat, Pig, Horse, Rabbit, Sheep,
产品应用
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
66kDa
细胞定位
细胞核 细胞浆 细胞膜
性 状
Lyophilized or Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthetic peptide derived from human FAM13C1:51-150/585
亚 型
IgG
纯化方法
affinity purified by Protein A
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed
PubMed
产品介绍
background: FAM13C1, also known as FAM13C, is a 585 amino acid protein that belongs to the FAM13 family. Existing as three alternatively spliced isoforms, the gene encoding FAM13C1 maps to human chromosome 10, which contains over 800 genes, 135 million nucleotides and makes up nearly 4.5% of the human genome. PTEN is an important tumor suppressor gene located on chromosome 10 and, when defective, causes a genetic predisposition to cancer development known as Cowden syndrome. The chromosome 10 encoded gene ERCC6 is important for DNA repair and is linked to Cockayne syndrome which is characterized by extreme photosensitivity and premature aging. Tetrahydrobiopterin deficiency and a number of syndromes involving defective skull and facial bone fusion are also linked to chromosome 10. As with most trisomies, trisomy 10 is rare and is deleterious.
Similarity: Belongs to the FAM13 family.
SWISS: Q8NE31
Gene ID: 220965
Database links: UniProtKB/Swiss-Prot: Q8NE31.2
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片
Sample: MCF-7 Cell (Human) Lysate at 40 ug Primary: Anti-FAM13C1 (bs-8215R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 66 kD Observed band size: 66 kD
Sample: Hela Cell (Human) Lysate at 30 ug Primary: Anti- FAM13C1 (bs-8215R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 66 kD Observed band size: 63 kD
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FAM13C1 Polyclonal Antibody, Unconjugated(bs-8215R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining