- ¥780 - 2200
- LMAI Bio
- LM-6982R
- 进口/国产
- 2025年11月04日
- WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg/Test （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat, Dog,
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- 详细信息
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-6982R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Neutrophil Elastase
- 抗体名：
中性粒细胞弹性蛋白酶ELANE抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Dog,
- 免疫原：
KLH conjugated synthetic peptide derived from human Neutrophil Elastase/ELANE:101-200/267
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg/Test （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
50ul 100ul 200ul
Neutrophil Elastase中性粒细胞弹性蛋白酶ELANE抗体
| 英文名称 | Neutrophil Elastase |
| 中文名称 | 中性粒细胞弹性蛋白酶ELANE抗体 |
| 别 名 | Bone Marrow Serine Protease; ELA 2; ELA2; ELANE; Elastase 2; Elastase-2; Elastase 2 neutrophil; Elastase neutrophil expressed; Elastase-2; ELNE_HUMAN; GE antibody Granulocyte derived elastase; HLE; HNE; Human leukocyte elastase; Leukocyte elastase; Leukocyte Elastase Precursor; Medullasin; NE; Neutrophil elastase; Neutrophill elastase; PMN E; PMN Elastase; Polymorphonuclear elastase; SCN1; Serine protease; SERP1. |
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Specific References (5) | bs-6982R has been referenced in 5 publications. [IF=5.23] Arecco, N., et al. "Elastase levels and activity are increased in dystrophic muscle and impair myoblast cell survival, proliferation and differentiation."Scientific Reports 6 (2016): 24708. IHC-P, WB ; Mouse. PubMed:27241590 [IF=2.26] Maekawa, Toshihiro, et al. "Prophylactic Effect of Lactobacillus pentosus strain S-PT84 on Candida Infection and Gastric Inflammation in a Murine Gastrointestinal Candidiasis Model [Errata]." Medical mycology journal 57.4 (2016): E81-E92. IHC-P ; Mouse. PubMed:27904074 [IF=2.35] Li, Guofu, et al. "The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats." International Journal of Molecular Medicine 38.3 (2016): 767-775. WB ; Rat. PubMed:27430552 [IF=4.22] Krishnan, Subramanian, et al. "Serotype O18 avian pathogenic and neonatal meningitis Escherichia coli strains employ similar pathogenic strategies for the onset of meningitis." Virulence 6.8 (2015): 777-786. IHC-P ; Mouse. PubMed:26407066 [IF=1.53] Jeffery, Unity, et al. "Dogs Cast NETs too: Canine neutrophil extracellular traps in health and immune-mediated hemolytic anemia." Veterinary Immunology and Immunopathology (2015). Dog. PubMed:26574161 |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 细胞生物 信号转导 细胞骨架 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg/Test （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 26kDa |
| 细胞定位 | 细胞浆 细胞外基质 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Neutrophil Elastase/ELANE:101-200/267 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009]. Function: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Subunit: Interacts with NOTCH2NL. Tissue Specificity: Bone marrow cells. DISEASE: Defects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Similarity: Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain. SWISS: P08246 Gene ID: 1991 Database links: Entrez Gene: 1991 Human Entrez Gene: 50701 Mouse Omim: 130130 Human SwissProt: P08246 Human SwissProt: Q3UP87 Mouse Unigene: 99863 Human Unigene: 262194 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample: Spinal cord (Mouse) Lysate at 40 ug Primary: Anti-Neutrophil Elastase (bs-6982R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 48 kD  Sample: HL60(Human) Cell Lysate at 30 ug Primary: Anti-Neutrophil Elastase (bs-6982R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 41 kD  Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Neutrophil Elastase (bs-6982R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 31/46 kD  Blank control Black line): A549 Primary Antibody (orange line): Rabbit Anti-Neutrophil Elastase/AF488 Conjugated antibody (bs-6982R-AF488) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-AF488(CST). Protocol The cells were fixed with 4% paraformaldehyde (10 min) , then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions followed by the antibody for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.  Blank control: A549(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green). |
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Neutrophil Elastase中性粒细胞弹性蛋白酶ELANE抗体
¥780 - 2200
