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- Biogems
- AF-300-05-500µg
- 美国
- 2025年07月16日
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500µg
靶点/蛋白质：SPLPITPVN ATCAIRHPC HNNLMNQIR SQLAQLNGS ANALFILYY TAQGEPFPN NLDKLCGPN VTDFPPFHA NGTEKAKLV ELYRIVVYL GTSLGNITR DQKILNPSA LSLHSKLNA TADILRGLL SNVLCRLCS KYHVGHVDV TYGPDTSGK DVFQKKKLG CQLLGKYKQ IIAVLAQAF
特异性：Mouse
官网链接：http://www.bio-gems.com/recombinant-proteins/animal-free-cytokines/animal-free-recombinant-human-lif.html
商品关键词：Animal-Free Recombinant Human LIF,AF-300-05-500µg,Biogems
简单描述：LIF is a pleiotrophic factor produced by multiple cell types including T cells, myelomoncytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant human LIF is a 19.6 kDa protein containing 180 amino acids residues including three disulfide bonds. Manufactured using all non-animal reagents.
Purity: Above 137% as determined by SDS-PAGE Analysis.
Biological Activity: Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
Cell Type: T Cells, Stem Cells
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文献和实验Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
Culturing Human Embryonic Stem Cells in Feeder-Free Conditions Sohyun L. McElroy1 and Renee A. Reijo Pera Center for Human Embryonic Stem Cell Research and Education, Institute for Stem Cell Biology and Regenerative Medicine
Maintenance of Human Embryonic Stem Cells in Animal Serum- and Feeder Layer-Free Culture Conditions
and future clinical application require an animal-free and well-defined culture system for hESCs, these conventional conditions would prevent the use of hESCs in human therapy. This chapter describes optional culture conditions based on either animal-free
Construction of Recombinant Human Cytomegalovirus
The use of reverse genetics to generate recombinant viruses allows the researcher to investigate the exact functional significance of particular viral genes during the virus life cycle, by means of their deletion or modification in the viral
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