ABP32; PEF; PEF protein with a long N terminal hydrophobic domain; PEF1A; PEFLIN; Penta EF hand domain containing protein 1.
规格价格
100ul/1380元 购买 200ul/2200元 购买 大包装/询价
说 明 书
100ul 200ul
研究领域
细胞生物 信号转导 细胞凋亡 转录调节因子 结合蛋白
抗体来源
Rabbit
克隆类型
Polyclonal
交叉反应
Human, Mouse, Rat, Pig, Cow, Horse, Sheep, Monkey,
产品应用
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
30kDa
细胞定位
细胞浆 细胞膜
性 状
Lyophilized or Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthetic peptide derived from human PEF1:121-230/284
亚 型
IgG
纯化方法
affinity purified by Protein A
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed
PubMed
产品介绍
background: PEF1 (penta-EF hand domain containing 1) is a Ca(2+)-binding protein that belongs to the penta-EF hand family which shares similarities to the apoptosis linked gene ALG-2. PEF1 heterodimerizes with PDCD6/ALG2 and dissociates from PDCD6/ALG2 in the presence of Ca(2+).
Subunit: Heterodimer; heterodimerizes with PDCD6. Dissociates from PDCD6 in presence of Ca(2+).
Subcellular Location: Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated in the presence of Ca(2+).
Similarity: Contains 5 EF-hand domains.
SWISS: Q9UBV8
Gene ID: 553115
Database links:
Entrez Gene: 553115 Human
Entrez Gene: 67898 Mouse
Entrez Gene: 297900 Rat
Omim: 610033 Human
SwissProt: Q9UBV8 Human
SwissProt: Q8BFY6 Mouse
SwissProt: Q641Z8 Rat
Unigene: 470417 Human
Unigene: 20818 Mouse
Unigene: 96097 Rat
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片
Sample: Intestine (Mouse) Lysate at 40 ug Primary: Anti-PEF1 (bs-6768R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD
Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).