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- 技术资料
- 供应商:
嵘崴达
- 库存:
需确认
- 英文名:
HIF-2-alpha overexpression 293T裂解物(whole cell)
- 规格:
100 µl
上海嵘崴达实业有限公司,专业经营生命科学领域的科研试剂、仪器和实验室消耗品,
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产品名称
HIF-2-alpha overexpression 293T裂解物(whole cell)
经测试应用
适用于: WB
-
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.01% 3,3-bis(3,5-dibromo-4-hydroxyphenyl)-3H-2,1λ6-benzoxathiole-1,1-dione, 2.3% 2-Mercaptoethanol, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine), 2% Sodium lauryl sulfate - 浓度
- 100 µl 浓度为 1.7 mg/ml
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文献和实验Transient transfection into 293T cells
Purpose Transient transfection into 293T cells is a convenient way to overexpress and obtain both cellular and extracellular (secreted or membrane) proteins. 293 is a human renal epithelial cell line which is transformed by adenovirus E1
Overexpression Models: Lentiviral Modeling of Brain Cancer
is described here. In this approach, a lenti?vector with lox P sites flanking the gene of interest (oncogene) is injected into mice expressing Cre recombinase under the control of a brain?specific promoter. The steps to perform cell?type/region?specific
Transient transfection into 293T cel
epithelial cell line which is transformed by adenovirus E1A gene product. 293T is a derivative which also express SV40 large T antigen, allowing episomal replication of plasmids containing the SV40 origin and early promoter region
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