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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Mouse
96T
货号 | EK0572 |
---|---|
产品名称 | Mouse CD30L ELISA Kit |
产品描述 | For quantitative detection of mouse CD30L in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, Q68-D239 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <6pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | TNFSF8 |
基因全名 | tumor necrosis factor (ligand) superfamily, member 8 |
Uniprot ID | P32972 |
指标别名 | CD153 antigen|CD30 antigen ligand|CD30 L|CD30 ligand|CD30L|CD30LG|MGC138144|TNFSF8|Tumor necrosis factor (ligand) superfamily member 8|Tumor necrosis factor ligand superfamily member 8 |
背景资料 | The human CD30L(CD153) gene is located at 9q33. It contains 4 exons and spans more than 17.1 kb. CD153 is expressed on the surface of B cells and this expression is upregulated upon CD154(CD40LG), IL4, and B-cell receptor engagement. In these cells, engagement of CD153 by T cell CD30 inhibits immunoglobulin class switch recognition as well as IgG, IgA, and IgE production, suggesting that this"reverse signaling" modulates the CD154-dependent switching of B cells into the pool producing IgG, IgA, and IgE. Additionally, recombinant human CD30L enhanced the proliferation of CD3 -activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines.The standard product used in this kit is recombinant mouse CD30L with the molecular mass of 30-45KDa. |
显色时间 | 25-30分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0572-CAP | 96-well plate precoated with anti- mouse CD30L antibody | 1 |
EK0572-ST | Lyophilized recombinant mouse CD30L standard | 10ng/tubex2 |
EK0572-DA | Biotinylated anti- mouse CD30L antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 35 | 254 | 445 | 38 | 267 | 477 |
Standard deviation | 2.24 | 16.51 | 34.26 | 2.58 | 21.09 | 43.4 |
CV(%) | 6.4% | 6.5% | 7.7% | 6.8% | 7.9% | 9.1% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.073 | 0.112 | 0.133 | 0.203 | 0.326 | 0.594 | 1.146 | 2.091 |
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