- ¥780 - 2200
- LMAI Bio
- LM-6703R
- 进口/国产
- 2026年01月06日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3ug/Test IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-6703R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
RAB7
- 抗体名：
RAS癌基因相关蛋白RAB7抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
- 免疫原：
KLH conjugated synthetic peptide derived from human RAB7:101-207/207
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3ug/Test IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
50ul 100ul 200ul
| 英文名称 | RAB7 |
| 中文名称 | RAS癌基因相关蛋白RAB7抗体 |
| 别 名 | CMT2B; Member RAS oncogene family; PSN; RAB7A; RAB7B; Ras associated protein RAB7; Ras related protein Rab7; Ras related protein Rab7a; RAB7A_HUMAN. |
 |
Specific References (1) | bs-6703R has been referenced in 1 publications. [IF=3.02] Naga, Shereen Hassan Aboul, et al. "Intracellular pathways following uptake of bevacizumab in RPE cells." Experimental Eye Research (2014). Human. PubMed:25533679 |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 肿瘤 细胞生物 信号转导 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3ug/Test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 23kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RAB7:101-207/207 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Members of the RAB family of RAS related GTP binding proteins are important regulators of vesicular transport and are located in specific intracellular compartments. RAB7 has been localized to late endosomes and shown to be important in the late endocytic pathway. In addition, it has been shown to have a fundamental role in the cellular vacuolation induced by the cytotoxin VacA of Helicobacter pylori. Function: Involved in late endocytic transport. Contributes to the maturation of phagosomes (acidification). Subunit: Interacts with RILP. Interacts with PSMA7. Interacts with RNF115. Interacts with FYCO1 Subcellular Location: Late endosome. Lysosome. Cytoplasmic vesicle, phagosome. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Tissue Specificity: Widely expressed; high expression found in skeletal muscle. DISEASE: Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B) [MIM:600882]; also known as hereditary motor and sensory neuropathy II (HMSN2). CMT2B is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. CMT2B is clinically characterized by marked distal muscle weakness and a high frequency of foot ulcers, infections and amputations of the toes. CMT2B inheritance is autosomal dominant. Similarity: Belongs to the small GTPase superfamily. Rab family. SWISS: P51149 Gene ID: 7879 Database links: Entrez Gene: 7879 Human Entrez Gene: 19349 Mouse Entrez Gene: 29448 Rat Omim: 602298 Human SwissProt: P51149 Human SwissProt: P51150 Mouse SwissProt: P09527 Rat Unigene: 741172 Human Unigene: 333233 Mouse Unigene: 486309 Mouse Unigene: 1425 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-RAB7 (bs-6703R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 18 kD  Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAB7) Polyclonal Antibody, Unconjugated (bs-6703R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-RAB7 Antibody, conjugated (bs-6703R-AF594) at 1:100 overnight at 4°C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.  Blank control: A431. Primary Antibody (green line): Rabbit Anti-RAB7 antibody (bs-6703R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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文献和实验Detection of Activated Rab7 GTPase with an Immobilized RILP Probe
The dynamic and coordinated exchange of multiple GTPases between the cytosol and the phagosome membrane represents a critical process during phagosome biogenesis. In particular, acquisition of Rab7 is crucial for progression to the stage
PNAS：RAS 抑制剂又添一员，新型蛋白模拟物或为癌症治疗
导读RAS 是肿瘤患者中最常见的致癌基因之一，目前包括 KRAS、NRAS 以及 HRAS 三种亚型。其中，NRAS 突变多见于黑色素瘤和急性髓系白血病，HRAS 突变多见于膀胱癌和头颈癌，而 KRAS 突变比其他两种更常见，最常发生在肺癌、胰腺癌以及结直肠癌。由于在癌症中的 Ras 蛋白经常发生突变，因此 Ras 突变成为了科学家们着力攻克的热点。RAS 曾被医学界称为「不可成药靶点」，科学家们在研究有效的 RAS 抑制剂方面已经摸索尝试了 30 余年，直至近几年才获得了重大进展。当前已经
Ras 信号通路 很多生长因子如EGF、PDGF、FGF等激活受体酪氨酸激酶后，通过中介分子可活化由原癌基因FaS编码的Ras蛋白 ，后者能进一步催化其底物蛋白的酪氨酸磷酸化反应，并引发蛋白磷酸化的级联反应，最终导致细胞的增殖效应。由于Ras蛋白为多种生长因子信号转导过程所共有，故把此信号转导途径称为Ras通路。 Ras蛋白是癌基因las的编码产物，人类有3种ras基因，即H―FaS、K―las和N―Fas，分布于不同染色体上，能编码蛋白质P21。所有的Ras―P21均有结合鸟
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