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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-5846R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
ADAM11
- 抗体名:
去整合素样金属蛋白酶11抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Cow, Sheep,
- 免疫原:
KLH conjugated synthetic peptide derived from human ADAM11:351-450/769
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | ADAM11 |
| 中文名称 | 去整合素样金属蛋白酶11抗体 |
| 别 名 | A disintegrin and metalloproteinase domain 11; ADA11_HUMAN; MDC; Metalloproteinase like, disintegrin like, and cysteine rich protein. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 信号转导 细胞骨架 细胞外基质 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Cow, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 60kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADAM11:351-450/769 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene represents a candidate tumor supressor gene for human breast cancer based on its location within a minimal region of chromosome 17q21 previously defined by tumor deletion mapping. [provided by RefSeq, Jul 2008]. Function: Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. Subunit: Can bind to LGI1 and LGI4. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed predominantly in brain. Slightly detected or not at all in other tissues. Post-translational modifications: The precursor is cleaved by a furin endopeptidase. Similarity: Contains 1 disintegrin domain. Contains 1 EGF-like domain. Contains 1 peptidase M12B domain. SWISS: O75078 Gene ID: 4185 Database links: Entrez Gene: 4185 Human Omim: 155120 Human SwissProt: O75078 Human Unigene: 6088 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 细胞外基质蛋白 |
| 产品图片 | ![]() Sample: A549 Cell (Human) Lysate at 40 ug Primary: Anti-ADAM11 (bs-5846R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD ![]() Sample: brain (Mouse) Lysate at 40 ug Primary: Anti-ADAM11 (bs-5846R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD |
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文献和实验蛋白的降解从而完成免疫沉淀实验非常关键。一般主要通过添加EDTA抑制金属蛋白酶,通过Protease Cocktail(多种蛋白酶抑制剂混合物)可以抑制蛋白酶。 5. 去垢剂对于免疫沉淀实验尤其是免疫共沉淀实验是一个非常关键的因素。不同的去垢剂种类和不同的去垢剂浓度主要通过影响以下三个因素来影响免疫沉淀效果: (1)细胞质/器膜的通透性:因为许多目的蛋白都定位在细胞器中,所以必须先将这些蛋白释放出来,抗体才能与之反应。 (2) 膜蛋白的释放:许多膜蛋白的构象对去垢剂种类和浓度非常敏感,因此针对这类
。6)滴加正常山羊血清封闭液,室温20分钟。甩去多余液体。7)滴加 Ⅰ 抗,室温1小时或者4℃过夜或者37℃1小时(4℃过夜后在37℃复温45分钟)。8)PBS洗三次每次2分钟。9)滴加生物素化二抗,20℃~37℃ 20 分钟。10)PBC 洗 3 次每次 2 分钟。11)滴加试剂SABC,20℃~37℃ 20 分钟。12)PBS 洗 4 次每次 5 分钟。13)DAB 显色:DAB 显色试剂盒或者自配显色剂显色(镜下掌握显色程度)。14)蒸馏水洗。苏木素复染 2 分钟、盐酸酒精分化。15)脱水、透明
1. 催化特点 ① 高效性 ② 专一性 键专一、基团专一、绝对或几乎绝对专一 ③ 可调节性 酶浓度调节、激素调节、共价修饰调节、限制性蛋白酶的水解作用与酶活调节、抑制剂的调节、反馈调节、金属离子和其他小分子化合物的调节。 2. 影响酶活因素 ①. 酶活测定方式 测定酶活,必须了解酶催化反应总的反应式,而且分析程序必须能够测定底物的消失或产物的生产。还需考虑辅助因子、酶的最适pH值和最适温度。 ②. 酶联测定法 过氧化物酶和它的共底物或辅酶必须过量,使酶联测定不属于限速步骤。 ③ 酶
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