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货期:1-2天
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MedChemExpress LLC
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100 rxns/500 rxns
| 规格: | 100 rxns | 产品价格: | ¥275.0 |
|---|---|---|---|
| 规格: | 500 rxns | 产品价格: | ¥911.0 |
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SYBR Green qPCR 预混液
MCE 国际站:SYBR Green qPCR 预混液
SYBR Green qPCR Master Mix 是一种 2× 浓度的即用型预混液,含有 qPCR 的所有组分, 只需加入样品 DNA,引物和水即可快速高效完成 qPCR 反应。
Description & Advantages:
The Super Hot-Start SYBR Green qPCR Master Mix is a ready-to-use qPCR cocktail. It contains a novel Taq DNA Polymerase, unique hot start reagents, optimized buffer, SYBR Green I, dNTPs, PCR Enhancer and PCR stabilizer. qPCR Master Mix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, passive reference dye (optional) and ddH2O.
MCE qPCR Master Mix can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability. Two separate tubes of ROX Reference Dye are included for use with instruments that require a high or low level of reference dye for rectify the error of fluorescence signals between different wells.
1. Ready to use.
2. Optimized buffer enhances specificity and reduces primer-dimer formation.
3. High sensitivity, specificity and reliability.
4. Passive reference dyes are provided for different qPCR instruments.
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文献和实验Taq Talk 荧光定量 PCR 系列课程之【qPCR 预混液的选择】一起来了解如何高效地选择合适的 qPCR 预混液
液为预先优化好的混合物,通常包含缓冲液、DNA聚合酶、dNTP、MgCl2及其他专利的添加剂。 一些专业人士坦言,对于多个指标,包括qPCR 效率、特异性、灵敏度、速度及耐受多个循环条件和样品类型,目前还尚未实现完美的平衡。大部分qPCR预混液能够在一些方面胜出,但很难是全部。如何选择最适合的那一个,小编下面给出了一些建议。 探针 还是染料? 当然,就多重分析而言,探针法是唯一选择。SYBR Green只适合单重反应。不过,SYBR Green的优势
SYBR Green Quantitative PCR Protocol
the relative values for your gene of interest’s change in expression. SYBR qPCR Quick Protocol StandardsØ Dilute the cDNA ~4-fold (e.g. 21μl sample + 59μl H2 O = 80μl)Ø Pool an appropriate amount from each sample to create standard 1 (i.e. 30μl x
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