- ￥6022
- Swant
- 瑞士
- 7697-200 µl.
- 2025年08月08日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
After reconstitution freeze in small aliquots (e.g. 1 µl) and keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
- 克隆性：
多克隆
- 适应物种：
Rabbit
- 供应商：
安诺伦（北京）生物科技有限公司
- 靶点：
Calretinin
- 规格：
200 µl.
研究领域：Calretinin
存储溶液：Lyophilized whole serum (no preservatives).
参考文献：1. Schwaller B., Buchwald P., Blümcke I., Celio M.R. and Hunziker W. (1994) Characterization of a polyclonal antiserum against the purified human recombinant calcium-binding protein calretinin. Cell Calcium 14: 639-648.2. Schiffmann S.N. et al (1999) Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin. PNAS, 96: 5257-5262.3. Gotzos V., Vogt P. and M.R. Celio (1995) Calretinin is a selective marker for malignant pleural mesotheliomas of the epithelial type. Pathol. Res. Pract. 192:137- 147.4. Doglioni, C. et al. (1996) Calretinin: a novel immunocytochemical marker for mesothelioma. Am. J. Surg. Pathol. 20:1037-1046.
应用：Immunohistochemistry: 1:2'000 - 1:5'000, on paraformaldehyde (4%) or formalin-fixed tissue, cryostate or paraffin-sections. Immunoblots: 1:1'000 - 1:2'000.For immunohistochemistry and immunoblots the titer was determined by using the avidin-biotin method. We recommend that the optimal dilutions be determined by titration experiments.
官网链接：https://www.swant.com/pdfs/Rabbit_anti_calretinin_7697.pdf
商品关键词：Rabbit anti-calretinin,7697-200 µl.,Swant
简单描述：The antiserum against calretinin is produced in rabbits by immunization with recombinant human calretinin containing a 6-his tag at the N-terminal. The antibody was evaluated for specificity and potency: a) by Biotin-Avidin labeling of cryostate-, vibratome- and paraffinsections of 4% paraformaldehyde fixed brains and b) by immunoenzymatic labelling of immunoblots.The product is a polyclonal antiserum against calretinin (1), a calcium-binding protein of the EF-hand family related to calbindin D-28k and calmodulin. The antibody reacts specifically with calretinin in tissue originating from human, monkey, rat, mouse, guinea pig, chicken and fish (Fig. 1). This antiserum does not cross-react with calbindin D-28k or other known calcium binding-proteins, as determined by immunoblots and by its distribution in the brain (Fig. 2).
Lot No: 1893-0114
Reconstitution: with 200 µl of bidistilled water.
Immunoblot: In Fig. 1, extracts of soluble proteins were isolated from whole brains of different species and separated by SDS-PAGE. In the Western blot the antiserum 7697 specifically recognizes a band of 29-30k Da.Fig. 1 Immunoblot of brain homogenates of various species with antiserum 7697. 1: Mouse, 2: Rat, 3. Guinea pig, 4. Rabbit, 5.Macaca fascicularis, 6. Zebrafish, 7. Chicken, 8. recombinant calretininImmunoblot of brain extracts: Antibody 7697 labels a subpopulation of neurons in the normal brain with high efficiency (Fig. 2a), but does not stain the brain of calretinin knock-out mice (Fig. 2b).
Product: Antiserum
uses: Calretinin belong probably to the class of “trigger” calcium binding-proteins. It occurs mainly in subpopulations of nerve cells and is an excellent markers for mesotheliomas(3,4).
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
Testing Rabbit Bleeds By Elisa
at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP) diluted in Blocking Buffer for 1 hr at RT (50 ul/well). Remove secondary antibody
Teratology Studies in the Rabbit
The rabbit is generally the non-rodent species or second species after the rat recommended by the regulatory authorities and is part of the package of regulatory reproductive studies for the detection of potential embryotoxic
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