胱抑素C elisa试剂盒，Cystatin C ELISa

Product name:

AuthentiKine™ Human Cystatin C ELISA Kit

Tests:

1 X 96 well plate

Sample type:

Serum, plasma, Cell culture supernatants, Urine, Saliva, Human milk

Assay type:

Sandwich

Sensitivity:

10.5 pg/mL

Range:

15.6 -500 pg/mL

Reacted Species:

Human

Tested applications:

Sandwich ELISA

Recovery:

Sample Type	Average	Range
Human plasma	89%	76%-95%
Cell culture supernatants	86%	73%-94%
Urine	97%	81%-107%
Saliva	89%	81%-98%
Human milk	94%	87%-100%

IntraAssay:

Sample	n	mean (pg/mL)	SD	CV%
1	20	108.1	8.3	7.6
2	20	203.8	16.4	8.0
3	20	348.3	23.5	6.7

InterAssay:

Sample	n	mean (pg/mL)	SD	CV%
1	24	100.3	6.2	6.2
2	24	211.9	18.7	8.8
3	24	370.6	26.2	7.1

Product overview:

KE00150 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Cystatin C ELISA kit is to be used to detect and quantify protein levels of endogenous Cystatin C. The assay recognizes human Cystatin C. An antibody specific for Cystatin C has been pre-coated onto the microwells. The Cystatin C protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for Cystatin C is added to detect the captured Cystatin C protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Storage Instructions:

All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

Synonyms:

ARMD11, CST3, Cystatin 3, cystatin C, Gamma trace, Post gamma globulin