MCF10A RFP-TUBA1B

MCF10A RFP-TUBA1B

General description

K-α1 tubulin/ tubulin α 1b (TUBA1B) is a microtubule protein. It is a ~50 kDa protein in its poly glycosylated, post-translational form. TUBA1B is present in cells as one of the six different isotypes. This gene is located on human chromosome 12q13.12.

This product was derived from ATCC Cat. No. CRL-10317.

MCF10A RFP-TUBA1B are fibrocystic disease, epithelial cells, from a human female (aged 36 years), with a RFP-tagged modification.

Application

MCF10A RFP-TUBA1B has been used in cell culture assays and western blot analysis.

MCF10A RFP-TUBA1B has been used to study the role of BRCA1 on the cell division axis.

This product is a human MCF10A cell line in which the genomic TUBA1B gene has been endogenously tagged with a Red Fluorescent Protein (RFP) gene using CompoZr^® Zinc Finger Nuclease technology. Integration resulted in endogenous expression of the fusion protein in which RFP is attached to the N-terminus of tubulin. Fluorescence imaging shows that the tubulin can polymerize to form the characteristic pattern of microtubules. Addition of paclitaxel causes the expected bundling of microtubules. This stable cell line was expanded from a single clone. The target’s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.


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