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- Thermo Scientific™
- 89874
- 2025年11月11日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Mitochondria Isolation Kit for Cultured Cells
- 供应商：
上海觅拓生物科技
- 保存条件：
常温
- 规格：
kit
描述
The Thermo Scientific Mitochondria Isolation Kit for Cultured Cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples in about 40 minutes.Features of the Mitochondria Isolation Kit for Cultured Cells:
• Fast—isolate intact mitochondria from 20 million cells in approximately 40 minutes (post-cell harvest)
• Flexible—process several samples simultaneously using the reagent-based method, or obtain highest possible yield with the Dounce homogenization method
• Optimizable—guidelines provided for optimizing purity vs. yield with the reagent-based method
• Benchtop-easy—isolation performed in a microcentrifuge tube
• Compatible—isolated mitochondria may be lysed with detergent or processed for almost any downstream application, including 2D electrophoresis
Isolation of mitochondria is typically a laborious process requiring single-sample processing with Dounce homogenization. The Mitochondria Isolation Kit uses a non-mechanical, reagent-based method that allows multiple (six) cultured cell samples to be processed concurrently in about 40 minutes. Cultured mammalian cell pellets are gently lysed using a proprietary formulation that results in maximum yield of mitochondria with minimal damage to integrity.
The product instructions describe guidelines for optimizing purity vs. yield parameters. The kit also offers an optimized Dounce homogenization procedure, which results in two-fold greater mitochondria recovery compared to the reagent-based method. Both methods use differential centrifugation to separate the mitochondrial and cytosolic fractions with a benchtop microcentrifuge and are completed in approximately 40 minutes (post-cell harvest). Once isolated, the mitochondria can be used in downstream applications such as apoptosis, signal transduction and metabolic studies, as well as to facilitate mitochondrial proteomics efforts.
规格
| Reagent Type: | Organelle Isolation, Subcellular Fractionation |
|---|---|
| Form: | Kit, Liquid |
| Product Size: | 115 mL |
内容及储存
Upon receipt store kit at 4°C. Product is shipped at ambient temperature.风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
2. Table top centrifuge capable at least 13,000 x g 3. 1.5 or 2 ml Nuclease-Free centrifuge tubes 4. 0.45 μm filter unit 实验步骤 Before starting: 1. Fresh or flash-frozen cultured cells can be used
The Analysis of tRNA Import Into Mammalian Mitochondria
Ribonucleic acid (RNA) import into mitochondria occurs in a variety of organisms. In mammalian cells, several small RNAs are imported in a natural manner; transfer RNAs (tRNAs) can be imported in an artificial way, following expression
. Prepare Dynabeads® Oligo (dT)25 as described below, and subsequently proceed with the mRNA isolation in "Direct mRNA Isolation Protocol". 2) Preparation of Lysate from Cultured Cells or Cell Suspension
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