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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-2922R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
Fetuin A/AHSG
- 抗体名:
胎球蛋白A
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep,
- 免疫原:
KLH conjugated synthetic peptide derived from human Alpha-2-HS-glycoprotein chain A.:201-300/367
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
50ul 100ul 200ul
| 英文名称 | Fetuin A/AHSG |
| 中文名称 | 胎球蛋白A |
| 别 名 | Fetuin-A; 59 kDa bone sialic acid containing protein; A2HS; Aa2 066; AHS; AHSG; Alpha 2 HS glycoprotein; Alpha 2 HS glycoprotein chain A; Alpha 2 Z globulin; Alpha 2HS glycoprotein; alpha2 Heremans Schmid glycoprotein; Asialofetuin; Ba alpha 2 glycoprotein; BSP; Countertrypin; FETUA; Fetuin A; Fetuin, mouse, homolog of ; FetuinA; Glycoprotein PP63; HSGA; pp63; Alpha-2-HS-glycoprotein chain A |
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Specific References (1) | bs-2922R has been referenced in 1 publications. [IF=3.99] Naito, Chisato, et al. "Facilitatory effects of fetuin-A on atherosclerosis."Atherosclerosis (2016). IHC-P ; Rat. PubMed:26828753 |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 免疫学 神经生物学 信号转导 细胞粘附分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 31/40kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Alpha-2-HS-glycoprotein chain A.:201-300/367 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure. [provided by RefSeq, Jul 2008] Function: Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. Subunit: Alpha-2-HS glycoprotein derives from this precursor, when the connecting peptide is cleaved off. The two chains A and B are held together by a single disulfide bond. Subcellular Location: Secreted. Tissue Specificity: Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins. Similarity: Belongs to the fetuin family. Contains 2 cystatin fetuin-A-type domains. SWISS: P02765 Gene ID: 197 Database links: Entrez Gene: 197 Human Entrez Gene: 26998 Human Omim: 138680 Human Omim: 605954 Human SwissProt: P02765 Human SwissProt: Q9UGM5 Human Unigene: 324746 Human Unigene: 81073 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Sample: liver (mouse)cell Lysate at 40 ug Primary: Anti- Fetuin A/AHSG (bs-2922R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31kD Observed band size: 34 kD |
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文献和实验人胎球蛋白-A ( Fetuin-A )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 Fetuin-A 单抗包被于酶标板上,标准品和样品中的 Fetuin-A与单抗结合,加入生物素化的抗人 Fetuin-A ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物
Deglycosylation of Glycoproteins Using Endoglycosidases
Enzymes, Substrates and Buffers: PNGase F1 Didansyl-fetuin glycopeptide (L-A-N(CHO)-AeC-S, triantennary), 100 mM Hepes, pH 8.6 Endo F1 Dansyl-N-(GlcNAc)2 (Man
Solid-Phase Assays of Receptor-Binding Specificity
or to peroxidase-labeled sialylglycoprotein fetuin (Fet-HRP); the apparent association constants of the virus–receptor complexes are calculated from the Scatchard plots of the binding data. In the fetuin-binding inhibition assay, the virus is incubated
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