Recombinant Mouse TIM-1/KIM-1/

Isolation and Culture of Mouse Pancreatic Islets for Ex Vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes

with a Ca2+ -free medium or with trypsin/dispase. The latter facilitates the introduction of recombinant or trappable probes and microimaging studies of, for example, changes in cytosolic-free Ca2+ concentration or the dynamics of individual organelles

Purification of Recombinant Poly(ADP-Ribose) Polymerases

, more than 11 mg of pure and highly active mouse PARP-2 can be obtained from 1 L of Sf9 insect cell culture.

Mouse keratinocyte cultures

  PRIMARY MOUSE KERATINOCYTE CULTURES Isolation of epidermal keratinocytes from neonatal mice is based on the protocol of Dlugosz et al., Methods Enzymol . 254:3-20 (1995). The epidermis from a newborn mouse should yield